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Estimation of nuclear DNA content in plants using flow cytometry
Four protocols for sample preparation (suspensions of intact cell nuclei) and the analysis of nuclear DNA amounts using FCM are presented and the most frequent problems encountered with plant material such as the interference of secondary metabolites are described.
Circumpolar phylogeography of Juncus biglumis (Juncaceae) inferred from AFLP fingerprints, cpDNA sequences, nuclear DNA content and chromosome numbers.
Chromosome numbers and genome size variation in Indian species of Curcuma (Zingiberaceae).
Close evolutionary relationships among some cytotypes are suggested based on the similarity in homoploid genome sizes and geographical grouping, and a new species combination, Curcuma scaposa (Nimmo), is proposed.
Genome size diversity in orchids: consequences and evolution.
It is revealed that Orchidaceae are currently the most variable angiosperm family with genome sizes ranging 168-fold (1C = 0.33-55.4 pg) and the importance of obtaining further genome size data given the considerable phylogenetic gaps which have been highlighted by the current study.
Flow cytometry with plant cells : analysis of genes, chromosomes and genomes
This work focuses on Flow Cytometry and Ploidy: Applications in Plant Systematics, Ecology and Evolutionary Biology, and the Consequences of Genome Size Variation across Plant Taxa.
Nuclear DNA amounts in Macaronesian angiosperms.
The authors' data support the hypothesis that the insular selection pressures in Macaronesia favour small C-values and genome sizes and both positive and negative correlations between infrageneric nuclear DNA amount variation and environmental conditions on Tenerife were also found in several genera.
The more the better? The role of polyploidy in facilitating plant invasions.
Polyploidy can be an important factor in species invasion success through a combination of 'pre-adaptation', whereby polyploid lineages are predisposed to conditions in the new range and, therefore, have higher survival rates and fitness in the earliest establishment phase.
Naturalized plants have smaller genomes than their non-invading relatives: a flow cytometric analysis of the Czech alien flora.
Institute of Botany, Academy of Sciences of the Czech Republic, CZ-252 43 Průhonice, Czech Republic, e-mail: email@example.com, firstname.lastname@example.org, email@example.com; Department of Botany,…
Genome size variation and species relationships in Hieracium sub-genus Pilosella (Asteraceae) as inferred by flow cytometry.
- J. Suda, A. Krahulcová, P. Trávnícek, Radka Rosenbaumová, Tomáš Peckert, F. Krahulec
- BiologyAnnals of botany
- 1 November 2007
Significant differences in genome size between hawkweed species from hybridogenous lineages involving the small-genome H. pilosella document the usefulness of nuclear DNA content as a supportive marker for reliable delineation of several of the most problematic taxa in Hieracium sub-genus Pilosella.