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The alphaviruses: gene expression, replication, and evolution.
The alphaviruses are a genus of 26 enveloped viruses that cause disease in humans and domestic animals. Mosquitoes or other hematophagous arthropods serve as vectors for these viruses. The completeExpand
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Structure of Dengue Virus Implications for Flavivirus Organization, Maturation, and Fusion
The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map. The virusExpand
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Visualization of membrane protein domains by cryo-electron microscopy of dengue virus
Improved technology for reconstructing cryo-electron microscopy (cryo-EM) images has now made it possible to determine secondary structural features of membrane proteins in enveloped viruses. TheExpand
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Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution.
The sequence of the entire RNA genome of the type flavivirus, yellow fever virus, has been obtained. Inspection of this sequence reveals a single long open reading frame of 10,233 nucleotides, whichExpand
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Processing of the envelope glycoproteins of pestiviruses.
The genomic RNA of pestiviruses is translated into a large polyprotein that is cleaved into a number of proteins. The structural proteins are N terminal in this polyprotein and include threeExpand
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Evolutionary Relationships and Systematics of the Alphaviruses
ABSTRACT Partial E1 envelope glycoprotein gene sequences and complete structural polyprotein sequences were used to compare divergence and construct phylogenetic trees for the genus Alphavirus. TreeExpand
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Conserved elements in the 3' untranslated region of flavivirus RNAs and potential cyclization sequences.
We have isolated a cDNA clone after reverse transcription of the genomic RNA of Asibi yellow fever virus whose structure suggests it was formed by self-priming from a 3'-terminal hairpin of 87Expand
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Production of infectious RNA transcripts from Sindbis virus cDNA clones: mapping of lethal mutations, rescue of a temperature-sensitive marker, and in vitro mutagenesis to generate defined mutants.
We constructed full-length cDNA clones of Sindbis virus that can be transcribed in vitro by SP6 RNA polymerase to produce infectious genome-length transcripts. Viruses produced from in vitroExpand
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Regulation of Sindbis virus RNA replication: uncleaved P123 and nsP4 function in minus-strand RNA synthesis, whereas cleaved products from P123 are required for efficient plus-strand RNA synthesis.
Nonstructural proteins of Sindbis virus, nsP1, nsP2, nsP3, and nsP4, as well as intermediate polyproteins, are produced from two precursor polyproteins, P123 and P1234, by a proteolytic enzymeExpand
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Polypeptide requirements for assembly of functional Sindbis virus replication complexes: a model for the temporal regulation of minus‐ and plus‐strand RNA synthesis.
Proteolytic processing of the Sindbis virus non‐structural polyproteins (P123 and P1234) and synthesis of minus‐ and plus‐strand RNAs are highly regulated during virus infection. Although theirExpand
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