Derivation of Oocytes from Mouse Embryonic Stem Cells
It is shown that mouse embryonic stem cells in culture can develop into oogonia that enter meiosis, recruit adjacent cells to form follicle-like structures, and later develop into blastocysts.
Pml Is Critical for Nd10 Formation and Recruits the Pml-Interacting Protein Daxx to This Nuclear Structure When Modified by Sumo-1
The findings identify the basic requirements for ND10 formation and suggest a dynamic mechanism for protein recruitment to these nuclear domains controlled by the SUMO-1 modification state of PML.
Niemann-Pick C1 disease gene: homology to mediators of cholesterol homeostasis.
Transfection of NP-C fibroblasts with wild-type NPC1 cDNA resulted in correction of their excessive lysosomal storage of LDL cholesterol, thereby defining the critical role of NPC1 in regulation of intracellular cholesterol trafficking.
Purification, characterization, and in vitro differentiation of cytotrophoblasts from human term placentae.
It is concluded that human cytotrophoblasts differentiate in culture and fuse to form functional syncytiotrophobic cells, similar to that of intact term placentae.
The Extra Domain A of Fibronectin Activates Toll-like Receptor 4*
Rec recombinant EDA, but not other recombinant fibronectin domains, activates human TLR4 expressed in a cell type (HEK 293 cells) that normally lacks this Toll-like receptor.
The pathophysiology and genetics of congenital lipoid adrenal hyperplasia.
- H. Bose, T. Sugawara, J. Strauss, W. Miller
- Medicine, BiologyNew England Journal of Medicine
- 19 December 1996
The congenital lipoid adrenal hyperplasia phenotype is the result of two separate events, an initial genetic loss of steroidogenesis that is dependent on steroidogenic acute regulatory protein and a subsequent loss of steroidsynthesis that is independent of the protein due to cellular damage from accumulated cholesterol esters.
Deficiency in catechol-O-methyltransferase and 2-methoxyoestradiol is associated with pre-eclampsia
The studies identify a genetic mouse model for pre-eclampsia and suggest that 2-ME may have utility as a plasma and urine diagnostic marker for this disease, and may also serve as a therapeutic supplement to prevent or treat this disorder.
Inflammation and Pregnancy
- J. Challis, C. Lockwood, L. Myatt, J. Norman, J. Strauss, F. Petraglia
- Biology, MedicineReproductive Sciences
- 15 September 2006
During pregnancy, the balance of Th1 (cell-mediated immunity) and Th2 (humoral immunity) cytokines is characterized by an initial prevalence of Th2 cytokines, followed by a progressive shift toward Th1 predominance late in gestation, that when is abnormal, may initiate and intensify the cascade of inflammatory cytokine production involved in adverse pregnancy outcomes.
Role of steroidogenic acute regulatory protein in adrenal and gonadal steroidogenesis.
In three unrelated individuals with this disorder, steroidogenic acute regulatory protein, which enhances the mitochondrial conversion of cholesterol into pregnenolone, was mutated and nonfunctional, providing genetic evidence that this protein is indispensable normal adrenal and gonadal steroidogenesis.
Thirty-seven candidate genes for polycystic ovary syndrome: strongest evidence for linkage is with follistatin.
- M. Urbanek, R. Legro, A. Dunaif
- Medicine, BiologyProceedings of the National Academy of Sciences…
- 20 July 1999
This study shows how a systematic screen of candidate genes can provide strong evidence for genetic linkage in complex diseases and can identify those genes that should have high (or low) priority for further study.