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Methods for binding cells to plastic: application to a solid-phase radioimmunoassay for cell-surface antigens.
Two methods are described for attaching cells to plastic plates such that they may be used for antibody binding assays. In the first method, lymphoid cells or erythrocytes were attached to the wellsExpand
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Tolerance induction in maturing B cells.
A two-stage tissue culture system was used to test the concept of clonal abortion as a mechanism for tolerance induction in B cells. In the first stage, neonatal spleen cells or bone marrow cellsExpand
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INHIBITION OF A T-CELL-DEPENDENT IMMUNE RESPONSE IN VITRO BY THYMOMA CELL IMMUNOGLOBULIN
Concentrated medium obtained from cultures of a continuous thymus-derived mouse lymphoma cell line (WEHI-22.1) was found to inhibit a T-cell-dependent (antidonkey red blood cell), but not aExpand
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Murine Monoclonal Antibodies against HLA Structures
The specificity of antibodies, encoded in their combining sites, is complementary to determinant groups of the immunogen. These determinants are tridimensional fractions of a larger structure.Expand
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LEUKOCYTE ANTIGENS IN RENAL TRANSPLANTATION: 1. THE PARADOX OF BLOOD TRANSFUSIONS IN RENAL TRANSPLANTATION
PATIENTS in terminal renal failure who are awaiting renal transplantation are generally maintained on frequent bremodialysis. This period of maintenance may last for several months, during which timeExpand
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Separation of human cells bearing HLA-DR antigens using a monoclonal antibody rosetting method.
A technique is described for enriching, from human blood, cells bearing HLA-DR antigens. The method depends on the use of monoclonal mouse antibody which reacts with HLA-DR structures. Cells to whichExpand
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Differentiation of lymphocytes in the mouse bone marrow. III. The adoptive response of bone marrow cells to a thymus cell-independent antigen.
The response of mouse spleen cells to the T cell-independent antigen dinitrophenylated polymer of flagellin (DNP—POL), has been studied using an adoptive transfer system, and compared with theExpand
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Prospective leucocyte typing in cadaver renal transplantation.
Abstract Leucocytes from donor and recipient were typed in the HL-A antigen system in twenty-seven renal allotransplants. In nineteen the findings were available at the time when the recipient wasExpand
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Hapten-specific B lymphocytes: enrichment, cloning, receptor analysis, and tolerance induction.
In this paper, we review experiments verifying clonal selection through the use of hapten-fractionated B lymphocytes of such uniformity that up to one cell in three can be induced to form anExpand
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Estimation of hapten-specific antibody-forming cell precursors in microcultures.
  • J. Stocker
  • Biology, Medicine
  • Immunology
  • 1 February 1976
The immune response of mouse spleen cells to hapten-conjugated polymer of flagellin (DNP-POL, NIP-POL) was studied using a microculture system. When increasing numbers of spleen cells were added to aExpand
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