Phylogeny and taxonomy of the Ophiostoma piceae complex and the Dutch elm disease fungi
Limitation fragment length polymorphisms of the ITS region are shown to be a convenient diagnostic tool for delimiting these species within the Ophiostoma piceae complex.
The root rot fungus Armillaria mellea introduced into South Africa by early Dutch settlers
- M. Coetzee, B. Wingfield, T. Harrington, J. Steimel, T. Coutinho, M. Wingfield
- Biology, MedicineMolecular Ecology
- 1 February 2001
Somatic compatibility tests and DNA fingerprinting with multilocus, microsatellite probes indicated that the Cape Town isolates were genetically identical and may have resulted from vegetative (clonal) spread from a single focus in the centre of the original Company Gardens (c. 1652).
Genetic variation in eastern North American and putatively introduced populations of Ceratocystis fimbriata f. platani
The genetic homogeneity of the fungus in Europe suggests that this population has gone through a recent genetic bottleneck, perhaps from the introduction of a single genotype, and supports the hypothesis that the pathogen was introduced to Europe through Naples, Italy during World War II.
Deletion of the MAT-2 mating-type gene during uni-directional mating-type switching in Ceratocystis
- R. Witthuhn, T. Harrington, B. Wingfield, J. Steimel, M. Wingfield
- BiologyCurrent Genetics
- 1 July 2000
The DNA sequence data for the C. eucalypti MAT-2 mating-type gene was increased to 1371-bp using TAIL-PCR and uneven PCR, representing a portion of the completeMAT-2 gene DNA sequence, supporting the hypothesis that the MAT-1 mating- type gene is deleted during uni-directional mating-types switching.
Development and characterization of microsatellite markers for the fungus Ceratocystis fimbriata
Microsatellite markers for C. fimbriata will be valuable for phylogenetic and population genetic studies, as well as for tracking accidental introductions of host-specialized forms of the pathogen.
Discovery of midgut genes for the RNA interference control of corn rootworm
The data indicate that dvssj1 and dvSSj2 are effective gene targets for the control of WCR using RNAi technology, by apparent suppression of production of their respective smooth septate junction membrane proteins located within the intestinal lining, leading to growth inhibition and mortality.
Genetic relationships among Leptographium terebrantis and the mycangial fungi of three western Dendroctonus bark beetles
Morphology, mitochondrial DNA (mtDNA) restriction fragment polymorphisms (RFLPs) and nuclear DNA (nDNA) fingerprinting were used to clarify relationships among the morphologically similar Ophiostoma…
Genetic variation in three Ceratocystis species with outcrossing, selfing and asexual reproductive strategies
Nuclear and mitochondrial markers were used to examine variation within three closely related species of tree pathogens with differing reproductive strategies, and selfing in C. virescens could explain an intermediate level of diversity when compared to the obligately outcrossing C. eucalypti and the asexual Ch.
Comparison of isozymes, rDNA spacer regions and MAT-2 DNA sequences as phylogenetic characters in the analysis of the Ceratocystis coerulescens complex
The phylogeny inferred from the MAT-2 HMG box sequences was found to be similar to the phylogeny based on the internal transcribed spacer (ITS) regions of the rDNA, thus supporting the hypothesis that the adaptation to gymnosperms was a single evolutionary event in the history of Ceratocystis.
Species Delimitation and Host Specialization of Ceratocystis laricicola and C. polonica to Larch and Spruce.
- T. Harrington, N. V. Pashenova, D. Mcnew, J. Steimel, M. Konstantinov
- Environmental SciencePlant Disease
- 1 April 2002
Analysis of isolates of C. laricicola and C. polonica from Europe, Russia, and Japan suggest that the two fungal species are very closely related and are distinguished primarily by their physiological specialization to the hosts of their bark beetle vectors.