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The 5'-nucleotidases as regulators of nucleotide and drug metabolism.
The 5'-nucleotidases are a family of enzymes that catalyze the dephosphorylation of nucleoside monophosphates and regulate cellular nucleotide and nucleoside levels. While the nucleoside kinasesExpand
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Tumor-promoting functions of adenosine.
  • J. Spychala
  • Biology, Medicine
  • Pharmacology & therapeutics
  • 1 August 2000
Tumor growth is a multifactorial process that, in addition to mutations leading to dysregulated expression of oncogenes and tumor suppressive genes, requires specific conditions that provide aExpand
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Mammalian 5′-Nucleotidases*
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Cloning of human adenosine kinase cDNA: sequence similarity to microbial ribokinases and fructokinases.
Adenosine kinase catalyzes the phosphorylation of adenosine to AMP and hence is a potentially important regulator of extracellular adenosine concentrations. Despite extensive characterization of theExpand
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Characterization and chromosomal localization of JAM-1, a platelet receptor for a stimulatory monoclonal antibody.
We have previously reported the purification and characterization of a 32 kDa platelet surface glycoprotein that is recognized by the stimulatory monoclonal antibody, F11. The cDNA has been clonedExpand
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Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation.
The regulation of adenosine kinase (AK) activity has the potential to control intracellular and interstitial adenosine (Ado) concentrations. In an effort to study the role of AK in Ado homeostasis inExpand
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The evidence for two opposite, ATP-generating and ATP-consuming, extracellular pathways on endothelial and lymphoid cells.
Extracellular purines are important signalling molecules in the vasculature that are regulated by a network of cell surface ectoenzymes. By using human endothelial cells and normal and leukaemicExpand
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Role of Estrogen Receptor in the Regulation of Ecto-5′-Nucleotidase and Adenosine in Breast Cancer
Purpose: The purpose is to understand the expression of ecto-5′-nucleotidase (eN), an adenosine producing enzyme with potential roles in angiogenesis, growth, and immunosuppression, in estrogenExpand
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Expression of ecto-5′-nucleotidase (eN, CD73) in cell lines from various stages of human melanoma
Ecto-5′-nucleotidase is a GPI-anchored enzyme localized in cell membrane lipid rafts. Although it is highly expressed in many tumour cells, its specific function during tumorigenesis is unclear. WeExpand
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ATP and phosphate reciprocally affect subunit association of human recombinant High Km 5'-nucleotidase. Role for the C-terminal polyglutamic acid tract in subunit association and catalytic activity.
IMP-specific, High Km 5'-nucleotidase (EC 3.1.3.5) is an ubiquitous enzyme, the activity of which is highly regulated by substrate, ATP, and inorganic phosphate. The cDNA encoding this enzyme hasExpand
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