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Argonaute2 Is the Catalytic Engine of Mammalian RNAi
Gene silencing through RNA interference (RNAi) is carried out by RISC, the RNA-induced silencing complex. RISC contains two signature components, small interfering RNAs (siRNAs) and Argonaute familyExpand
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Polycomb Proteins Targeted by a Short Repeat RNA to the Mouse X Chromosome
To equalize X-chromosome dosages between the sexes, the female mammal inactivates one of her two X chromosomes. X-chromosome inactivation (XCI) is initiated by expression of Xist, a 17-kb noncodingExpand
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Crystal Structure of Argonaute and Its Implications for RISC Slicer Activity
Argonaute proteins and small interfering RNAs (siRNAs) are the known signature components of the RNA interference effector complex RNA-induced silencing complex (RISC). However, the identity ofExpand
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Purified Argonaute2 and an siRNA form recombinant human RISC
Genetic, biochemical and structural studies have implicated Argonaute proteins as the catalytic core of the RNAi effector complex, RISC. Here we show that recombinant, human Argonaute2 can combineExpand
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The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes
RISC, the RNA-induced silencing complex, uses short interfering RNAs (siRNAs) or micro RNAs (miRNAs) to select its targets in a sequence-dependent manner. Key RISC components are Argonaute proteins,Expand
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On the Convergent Evolution of Animal Toxins
BgK is a K+ channel-blocking toxin from the sea anemone Bunodosoma granulifera It is a 37-residue protein that adopts a novel fold, as determined by NMR and modeling. An alanine-scanning-basedExpand
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Structural basis of protein complex formation and reconfiguration by polyglutamine disease protein Ataxin-1 and Capicua.
Spinocerebellar ataxia type 1 (SCA1) is a dominantly inherited neurodegenerative disease caused by polyglutamine expansion in Ataxin-1 (ATXN1). ATXN1 binds to the transcriptional repressor CapicuaExpand
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Argonaute and RNA--getting into the groove.
RNAi has made an enormous impact on biology in a very short period of time. It became an extraordinarily useful and simple tool for gene silencing, even as its fascinating mechanism was graduallyExpand
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Human Argonaute 2 Has Diverse Reaction Pathways on Target RNAs.
Argonaute is a key enzyme of various RNA silencing pathways. We use single-molecule fluorescence measurements to characterize the reaction mechanisms of the core-RISC (RNA-induced silencing complex)Expand
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Huntingtin facilitates polycomb repressive complex 2
Huntington's disease (HD) is caused by expansion of the polymorphic polyglutamine segment in the huntingtin protein. Full-length huntingtin is thought to be a predominant HEAT repeat α-solenoid,Expand
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