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Structure and physiology of the mammalian mitotic spindle.
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CONFOCAL ANALYSIS OF PRIMARY CILIA STRUCTURE AND COLOCALIZATION WITH THE GOLGI APPARATUS IN CHONDROCYTES AND AORTIC SMOOTH MUSCLE CELLS
Detyrosinated and acetylated α‐tubulins represent a stable pool of tubulin typically associated with microtubules of the centrosome and primary cilium of eukaryotic cells. Although primaryExpand
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UV microbeam irradiations of the mitotic spindle. II. Spindle fiber dynamics and force production
Metaphase and anaphase spindles in cultured newt and PtK1 cells were irradiated with a UV microbeam (285 nM), creating areas of reduced birefringence (ARBs) in 3 s that selectively either severed aExpand
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Loss of mitotic centrosomal microtubule initiation capacity at the metaphase-anaphase transition.
The microtubule initiation capacity of the mitotic centrosome was studied in PtK1 cells by using the highly reversible microtubule inhibitor nocodazole. Cells blocked with nocodazole at any stageExpand
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Initiation and growth of microtubules from mitotic centers in lysed mammalian cells
Metaphase PtK1 cells, lysed into polymerization-competent microtubule protein, maintain a spindle which will gain or lose birefringence depending on the concentration of disassembled tubulin subunitsExpand
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Cytochalasin J affects chromosome congression and spindle microtubule organization in PtK1 cells.
PtK1 cells were treated with 10 micrograms/ml cytochalasin J (CJ) for 15 min at various stages of mitosis. When applied at nuclear envelope breakdown (NEB) chromosome congression was blocked orExpand
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Analysis of spindle microtubule organization in untreated and taxol‐treated PtK1 cells.
Taxol, a microtubule stabilizing agent, has been used to study changes in spindle microtubule organization during mitosis. PtK1 cells have been treated with 5 μg/ml taxol for brief periods toExpand
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An innovative fixative for cytoskeletal components allows high resolution in colocalization studies using immunofluorescence techniques
Using a new fixation solution, CytoSkelFix, it is now possible to obtain superior fixation and thus resolution of cytoskeletal components using immunofluorescence and fluorescence microscopy. ThisExpand
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Both actin and myosin inhibitors affect spindle architecture in PtK1 cells: does an actomyosin system contribute to mitotic spindle forces by regulating attachment and movements of chromosomes in
Immunocytochemical techniques are used to analyze the effects of both an actin and myosin inhibitor on spindle architecture in PtK1 cells to understand why both these inhibitors slow or blockExpand
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THE EFFECT OF CYTOCHALASIN J ON KINETOCHORE STRUCTURE IN PTK1CELLS IS MITOTIC CYCLE DEPENDENT
Mitotic PtK1cells were arrested in mitosis with nocodazole to determine the effect of cytochalasin J (CJ) on kinetochore structure in arrested and nocodazole‐released cells. In previous studies itExpand
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