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Causes of proteolytic degradation of secreted recombinant proteins produced in methylotrophic yeast Pichia pastoris: case study with recombinant ovine interferon-tau.
It was observed that during fermentative production of recombinant ovine interferon-tau (r-oIFN-tau) in Pichia pastoris, a secreted recombinant protein, the protein was degraded increasingly after 48Expand
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Optimazation of Submerged Culture Conditions for Exo-Biopolymer Production by Paecilomyces japonica
Optimization of submerged culture conditions for the production of exo-biopolymer from Paecilomyces japonica was studied. Maltose, yeast extract, and potassium phosphate were the most suitableExpand
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Changes in morphology of Paecilomyces japonica and their effect on broth rheology during production of exo-biopolymers
Abstract. The influence of Paecilomyces japonica pellet morphology on fermentation broth rheology and exo-biopolymer production was investigated in a 5-l jar fermenter. Rapid formation of pellets wasExpand
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  • Open Access
Enhancement of protein secretion in Pichia pastoris by overexpression of protein disulfide isomerase
A potential vaccine candidate, Necator americanus secretory protein (Na‐ASP1), against hookworm infections, has been expressed in Pichia pastoris. Na‐ASP1, a 45 kDa protein containing 20 cysteines,Expand
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Maximization of Production of Secreted Recombinant Proteins in Pichia pastoris Fed‐Batch Fermentation
Pontryaginapos;s Maximum Principle has been applied for optimization of secreted proteins from Pichia pastoris fed‐batch fermentation. The objective of this work is to maximize the total accumulatedExpand
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  • Open Access
Aqueous two-phase: the system of choice for extractive fermentation
Abstract Extractive fermentation in aqueous two-phase systems is a meaningful approach to overcome low product yield in a conventional fermentation process, and by proper design of the two-phaseExpand
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Pichia pastoris fermentation optimization: energy state and testing a growth-associated model
A growth-associated model was applied to the production of recombinant ovine interferon-τ (rOvIFN-τ) with Pichia pastoris for the purpose of manufacturing preclinical and clinical active material.Expand
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Improved Production of Recombinant Ovine Interferon‐τ by Mut+ Strain of Pichiapastoris Using an Optimized Methanol Feed Profile
Recombinant ovine interferon‐τ (r‐oIFN‐τ) production by Pichia pastoris was studied using methanol as the sole carbon source during induction. The cells were grown on glycerol up to a certain cellExpand
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Acceptor reactions of a novel transfructosylating enzyme from Bacillus sp.
Many different oligosaccharides were produced by transferring the fructose residue of sucrose to maltose, cellobiose, lactose and sucrose (self-transfer), where their yields of fructosylated acceptorExpand
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Purification and scale-up of a recombinant heavy chain fragment C of botulinum neurotoxin serotype E in Pichia pastoris GS115.
A recombinant C-terminus heavy chain fragment from botulinum neurotoxin serotype E (BoNT/E) is proposed as a vaccine against the serotype E neurotoxin. This fragment, rBoNTE(Hc), was producedExpand
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