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Transfer of Sulfur from IscS to IscU during Fe/S Cluster Assembly*
Surface plasmon resonance studies and isothermal titration calorimetry measurements revealed that IscU binds to IscS with high affinity (K d ∼2 μm) in support of a direct transfer mechanism, and suggested that the C-terminal region of IsCS may be important for binding Isc U.
Thermodynamic prediction of protein neutrality.
A simple theory that uses thermodynamic parameters to predict the probability that a protein retains the wild-type structure after one or more random amino acid substitutions is presented and provides a basis for interpreting the response of proteins to substitutions in protein engineering applications.
Library analysis of SCHEMA‐guided protein recombination
The computational algorithm SCHEMA was developed to estimate the disruption caused when amino acid residues that interact in the three‐dimensional structure of a protein are inherited from different
Interaction of the iron-sulfur cluster assembly protein IscU with the Hsc66/Hsc20 molecular chaperone system of Escherichia coli.
A direct and specific role for the Hsc66/Hsc20 chaperone system in functioning with isc gene components for the assembly of iron-sulfur cluster proteins is established.
Hsc66 Substrate Specificity Is Directed toward a Discrete Region of the Iron-Sulfur Cluster Template Protein IscU*
It is suggested that Hsc66 will not bind LPPVK motifs with high affinity in vivo unless they are in the context of native IscU and can be directed to H sc66 by Hsc20.
Hsc66 and Hsc20, a new heat shock cognate molecular chaperone system from Escherichia coli
It is suggested that Hsc66 and Hsc20 comprise a molecular chaperone system similar to the prokaryotic DnaK/DnaJ and eukaryotic hsp70/hsp40 systems.
The Hsc66-Hsc20 Chaperone System inEscherichia coli: Chaperone Activity and Interactions with the DnaK-DnaJ-GrpE System
Results suggest that Hsc66-Hsc20 and DnaK-DnaJ-GrpE comprise separate molecular chaperone systems with distinct, nonoverlapping cellular functions.
On the conservative nature of intragenic recombination.
It is shown that, among beta-lactamase variants containing the same number of amino acid substitutions, variants created by recombination retain function with a significantly higher probability than those generated by random mutagenesis.
Site-directed protein recombination as a shortest-path problem.
A computational algorithm is developed to enrich these libraries in folded proteins while maintaining an appropriate level of diversity for evolution by comparing these optima for different sets of parents to rapidly determine which parents yield the lowest energy libraries.
Biochar and microbial signaling: production conditions determine effects on microbial communication.
Findings provide the first direct evidence that biochars elicit a range of effects on gene expression dependent on intercellular signaling, implicating the method of biochar preparation as a parameter that could be tuned to regulate microbial-dependent soil processes, like nitrogen fixation and pest attack of root crops.