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Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression
Multiple, complex molecular events characterize cancer development and progression. Deciphering the molecular networks that distinguish organ-confined disease from metastatic disease may lead to the
Site-directed mutations in fungal laccase: effect on redox potential, activity and pH profile.
Although the redox potentials were not significantly altered, the Km, kcat and fluoride inhibition of the laccases were greatly changed by the mutations, interpreted as possible mutation-induced structural perturbations on the molecular recognition between the reducing substrate and laccase and on the electron transfer from the substrate to the type-1 Cu centre.
Biochemical Analysis of Recombinant Fungal Mutanases
Nucleotide sequence analysis shows that Trichoderma harzianum and Penicillium purpurogenum α1,3-glucanases (mutanases) have homologous primary structures, and the catalytic domains and the binding domains were assigned to a new family of glycoside hydrolases and to anew family of carbohydrate-binding domains, respectively.
Kluyveromyces as a Host for Heterologous Gene Expression: Expression and Secretion of Prochymosin
It is concluded that the efficient synthetic and secretory capacity of this heterologous protein is a property of the yeast Kluyveromyces, and led to the development of a large scale production process for chymosin.
Molecular preservation by extraction and fixation, mPREF: a method for small molecule biomarker analysis and histology on exactly the same tissue
The Molecular Preservation by Extraction and Fixation (mPREF) method allows for the analysis of small molecule biomarkers from exactly the same tissue that is processed for histopathology.
The alcohol dehydrogenase system in the yeast, Kluyveromyces lactis
ADH activities resolved by gel electrophoresis revealed several ADH isozymes which are differently expressed in K. lactis cells depending on the carbon source.
Gene discovery and gene function assignment in filamentous fungi
It is shown that insertions can create alterations in gene expression and be used to rapidly identify and mutate genes and an amino acid oxidation pathway in two important fungal phytopathogens is investigated.
Efficient gene identification and targeted gene disruption in the wheat blotch fungus Mycosphaerella graminicola using TAGKO
The application of genome-wide TAGKO to the wheat blotch fungus Mycosphaerella graminicola is reported, including the successful development of electroporation-based transformation for this fungus.