The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
- J. Shine, L. Dalgarno
- BiologyProceedings of the National Academy of Sciences…
- 1 April 1974
It is suggested that this region of the RNA is able to interact with mRNA and that the 3'-terminal U-U-A(OH) is involved in the termination of protein synthesis through base-pairing with terminator codons.
Sequence and expression of human estrogen receptor complementary DNA.
A complementary DNA clone containing the entire translated portion of the messenger RNA for the estrogen receptor from MCF-7 human breast cancer cells was sequenced and then expressed in Chinese hamster ovary cells to give a functional protein, which suggests that steroid receptor genes and the avian erythroblastosis viral oncogene are derived from a common primordial gene.
Cloning and expression of full-length cDNA encoding human vitamin D receptor.
- A. Baker, D. McDonnell, B. O’Malley
- BiologyProceedings of the National Academy of Sciences…
- 1 May 1988
Sequence comparisons demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.
Determinant of cistron specificity in bacterial ribosomes
- J. Shine, L. Dalgarno
- BiologyNature
- 6 March 1975
Complementarity relationships between this sequence and a purine-rich tract in the ribosome binding site of different bacterial mRNAs suggest that the 3′-end of 16S RNA determines the intrinsic capacity of ribosomes to translate a particular cistron.
Rapid screening for symbiotic mutants of Rhizobium and white clover
- B. Rolfe, P. Gresshoff, J. Shine
- Biology
- 1 September 1980
Relaxin gene expression in human ovaries and the predicted structure of a human preprorelaxin by analysis of cDNA clones.
Evidence is presented that the human genome possesses an additional relaxin‐related gene (designated human relaxin gene H2) which appears to be selectively expressed in the ovary during pregnancy and which encodes an authentic human Relaxin.
Rat insulin genes: construction of plasmids containing the coding sequences.
- A. Ullrich, J. Shine, H. Goodman
- BiologyScience
- 17 June 1977
Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the…
ApaI dimorphism at the human vitamin D receptor gene locus.
- J. Faraco, N. Morrison, A. Baker, J. Shine, P. Frossard
- BiologyNucleic Acids Research
- 11 March 1989
This probe was isolated from a human breast cancer cell line (T47D) cDNA library, and cloned as an EcoRI fragment into pGEM (1) and detects four invariant bands and a single two-allele polymorphism.
Protein-coupled Receptors
‘Seven transmembrane’ receptors are the site of action of the majority of clinically useful drugs, and there is also growing evidence that spontaneous mutations of the genes encoding such receptors may lead to a number of pathological conditions.
Primary structure and evolution of rat growth hormone gene.
- A. Barta, R. Richards, J. Baxter, J. Shine
- BiologyProceedings of the National Academy of Sciences…
- 1 August 1981
The rat growth hormone gene was isolated on a cloned 11.4-kilobase EcoRI-generated DNA fragment from a bacteriophage "library" of chromosomal DNA and the complete primary structure of the gene and the 5' and 3'-flanking regions was determined.
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