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The complete genome sequence of the Gram-positive bacterium Bacillus subtilis
TLDR
Bacillus subtilis is the best-characterized member of the Gram-positive bacteria, indicating that bacteriophage infection has played an important evolutionary role in horizontal gene transfer, in particular in the propagation of bacterial pathogenesis. Expand
Essential Bacillus subtilis genes
TLDR
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, a systematic inactivation of Bacillus subtilis genes was carried out and most genes involved in the Embden–Meyerhof–Parnas pathway are essential. Expand
Regulation and Characterization of a Newly Deduced Cell Wall Hydrolase Gene (cwlJ) Which Affects Germination of Bacillus subtilis Spores
TLDR
The predicted amino acid sequence of Bacillus subtilis ycbQ (renamed cwlJ) exhibits high similarity to those of the deduced C-terminal catalytic domain of SleBs, the specific cortex-hydrolyzing enzyme of B. subtilIs, which indicated that it is transcribed by EsigmaE RNA polymerase. Expand
Synthetic Lethality of the lytE cwlO Genotype in Bacillus subtilis Is Caused by Lack of d,l-Endopeptidase Activity at the Lateral Cell Wall
TLDR
This study demonstrated that the D,L-endopeptidase activity at the lateral cell wall is essential for cell proliferation and construction of N-terminal and C-Terminal domain-swapped enzymes of LytE, LytF, CwlS, and CwlO revealed that localization was dependent on the N- terminal domains. Expand
The CitST two‐component system regulates the expression of the Mg‐citrate transporter in Bacillus subtilis
TLDR
It is concluded that the expression of citM is positively regulated by the CitST system and negatively regulated by CcpA. Expand
Regulation of a New Cell Wall Hydrolase Gene,cwlF, Which Affects Cell Separation in Bacillus subtilis
TLDR
Bacillus subtilis produces a 35-kDa cell wall hydrolase, CwlF, which exhibits high sequence similarity to the p60 proteins of Listeria species, NlpC proteins of Escherichia coli and Haemophilus influenzae, and Enp2 protein of Bacillus sphaericus, and its sequence is completely identical to that of the internal region of the papQ gene product. Expand
Localization of the Vegetative Cell Wall Hydrolases LytC, LytE, and LytF on the Bacillus subtilis Cell Surface and Stability of These Enzymes to Cell Wall-Bound or Extracellular Proteases
TLDR
It is observed that cells producing LytC-3xFLAG are uniformly coated with the fusion protein after the middle of the exponential growth phase, which supports the suggestion that LyTC is a major autolysin that is not associated with cell separation. Expand
Functional Analysis of the YvrGHb Two-Component System of Bacillus subtilis: Identification of the Regulated Genes by DNA Microarray and Northern Blot Analyses
TLDR
The results indicate that the YvrGHb system is important to maintain the overall state of the cell surface through transcriptional regulation. Expand
spoIVH (ykvV), a Requisite Cortex Formation Gene, Is Expressed in Both Sporulating Compartments of Bacillus subtilis
TLDR
It is suggested that ykvV is capable of being transported by the putative Sec-type signal sequence into the space between the double membranes surrounding the forespore, and should be renamed spoIVH. Expand
Bacillus minimum genome factory: effective utilization of microbial genome information
TLDR
The MG1M strain had cellulase and protease productivity comparable with that of the B. subtilis 168 strain, thus demonstrating that genome reduction does not contribute to a negative influence on enzyme productivity. Expand
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