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Spatial partitioning of the regulatory landscape of the X-inactivation centre
In eukaryotes transcriptional regulation often involves multiple long-range elements and is influenced by the genomic environment. A prime example of this concerns the mouse X-inactivation centre… Expand
Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination.
Structured illumination microscopy is a method that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit by using spatially structured illumination… Expand
Interphase chromosomes undergo constrained diffusional motion in living cells
BACKGROUND Structural studies of fixed cells have revealed that interphase chromosomes are highly organized into specific arrangements in the nucleus, and have led to a picture of the nucleus as a… Expand
Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate.
n-Prop]yl gallate (0.1 to 0.25 molar, in glycerol) reduces by a factor of 10 the rate of fading of fluorescence of cell structures labeled with tetramethylrhodamine or fluorescein-conjugated… Expand
Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy
Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial… Expand
Fluorescence microscopy in three dimensions.
The combination of the specificity provided by fluorescence microscopy and the ability to quantitatively analyze specimens in three dimensions allows the fundamental organization of cells to be… Expand
Polarization of chemoattractant receptor signaling during neutrophil chemotaxis.
- G. Servant, O. Weiner, P. Herzmark, T. Balla, J. Sedat, H. Bourne
- Biology, Medicine
- 11 February 2000
Morphologic polarity is necessary for chemotaxis of mammalian cells. As a probe of intracellular signals responsible for this asymmetry, the pleckstrin homology domain of the AKT protein kinase (or… Expand
Mitosis in living budding yeast: anaphase A but no metaphase plate.
Chromosome movements and spindle dynamics were visualized in living cells of the budding yeast Saccharomyces cerevisiae. Individual chromosomal loci were detected by expression of a protein fusion… Expand
Direct Evidence of a Role for Heterochromatin in Meiotic Chromosome Segregation
We have investigated the mechanism that enables achiasmate chromosomes to segregate from each other at meiosis I in D. melanogaster oocytes. Using novel cytological methods, we asked whether… Expand
Time-Lapse Microscopy Reveals Unique Roles for Kinesins during Anaphase in Budding Yeast
The mitotic spindle is a complex and dynamic structure. Genetic analysis in budding yeast has identified two sets of kinesin-like motors, Cin8p and Kip1p, and Kar3p and Kip3p, that have overlapping… Expand