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A Flux Model of Glycolysis and the Oxidative Pentosephosphate Pathway in Developing Brassica napus Embryos*
The observed distribution of label in cytosolic and plastidic metabolites indicated that key intermediates of glycolysis and OPPP have similar labeling in these two compartments, suggesting rapid exchange of metabolites between these compartments compared with net fluxes into end products. Expand
Biosynthesis of isoprenoids in higher plant chloroplasts proceeds via a mevalonate‐independent pathway
The dichotomy in isoprenoid biosynthesis in higher plants allows a reasonable interpretation of previous odd and inconclusive results concerning the biosynthesis of chloroplast isopranoids, which so far had mainly been interpreted in the frame of models using compartmentation of the mevalonate pathway. Expand
Rubisco without the Calvin cycle improves the carbon efficiency of developing green seeds
In developing embryos of Brassica napus L. (oilseed rape), Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase) acts without the Calvin cycle and in a previously undescribed metabolic context to increase the efficiency of carbon use during the formation of oil. Expand
Two independent biochemical pathways for isopentenyl diphosphate and isoprenoid biosynthesis in higher plants
In higher plants, there exist two separate and biochemically different IPP biosynthesis pathways: (1) the novel alternative GAP/pyruvate pathway apparently bound to the plastidic compartment and (2) the classical cytoplasmic acetate/mevalonate pathway. Expand
Arabidopsis Genes Involved in Acyl Lipid Metabolism. A 2003 Census of the Candidates, a Study of the Distribution of Expressed Sequence Tags in Organs, and a Web-Based Database1
The genome of Arabidopsis has been searched for sequences of genes involved in acyl lipid metabolism. Over 600 encoded proteins have been identified, cataloged, and classified according to predictedExpand
The Capacity of Green Oilseeds to Utilize Photosynthesis to Drive Biosynthetic Processes1
It is demonstrated that even the low level of light reaching seeds plays a substantial role in activating light-regulated enzymes, increasing fatty acid synthesis, and potentially powering refixation of CO2. Expand
Isoprenoid biosynthesis in eukaryotic phototrophs: a spotlight on algae.
Current views on the biochemistry and genetics of the core isoprenoid metabolism in land plants and in the major algal phyla are compared and some of the most pressing open questions are highlighted. Expand
Oil accumulation is controlled by carbon precursor supply for fatty acid synthesis in Chlamydomonas reinhardtii.
The results suggest that carbon availability is a key metabolic factor controlling oil biosynthesis and carbon partitioning between starch and oil in Chlamydomonas. Expand
Mitochondrial Metabolism in Developing Embryos of Brassica napus*♦
The metabolism of developing plant seeds is directed toward transforming primary assimilatory products (sugars and amino acids) into seed storage compounds and metabolic fluxes were determined in developing embryos of Brassica napus to understand the role of mitochondria. Expand
Analysis of Metabolic Flux Phenotypes for Two Arabidopsis Mutants with Severe Impairment in Seed Storage Lipid Synthesis1[W][OA]
Flux analysis in cultured embryos revealed changes in nutrient uptakes and fluxes into biomass as well as an increase in tricarboxylic acid cycle activity for both mutations. Expand