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Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity
A Ti plasmid mutant was constructed in which all the on‐cogenic functions of the T‐DNA have been deleted and replaced by pBR322. This Ti plasmid, pGV3850, still mediates efficient transfer andExpand
Isolation of a dual plant promoter fragment from the Ti plasmid of Agrobacterium tumefaciens
The two most abundant transcripts derived from TR‐DNA within plant cells transformed by an octopine strain of Agrobacterium tumefaciens arise from divergent transcription, both originating within anExpand
Chimeric genes as dominant selectable markers in plant cells
Opine synthases are enzymes produced in dicotyledonous plants as the result of a natural gene transfer phenomenon. Agrobacteria contain Ti plasmids that direct the transfer, stable integration andExpand
Transfer of Ti plasmids between Agrobacterium strains by mobilisation with the conjugative plasmid RP4
SummaryThe P type conjugative plasmid RP4 has been shown to be able to promote the transfer of the Agrobacterium Ti-plasmid. The results provide additional evidence that agrocin 84 sensitivity,Expand
Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability
THE gram-negative bacterium Agrobacterium tumefaciens induces crown gall tumours in many, mostly dicotyledonous, plants. Zaenen et al.1 demonstrated the presence of one or more large plasmids in aExpand
Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector
Foreign genes introduced into plant cells with Ti-plasmid vectors are not expressed. We have constructed an expression vector derived from the promoter sequence of nopaline synthase, and haveExpand
RecA protein stimulates homologous recombination in plants.
A number of RecA-like proteins have been found in eukaryotic organisms. We demonstrate that the prokaryotic recombination protein RecA itself is capable of interacting with genomic homologous DNA inExpand
Expression of foreign genes in regenerated plants and in their progeny
Chimeric genes comprised of the nopaline synthase promoter and bacterial coding sequences specifying resistance to kanamycin, chloramphenicol or methotrexate, were inserted into the non‐oncogenic TiExpand
High rates of Ac/Ds germinal transposition in Arabidopsis suitable for gene isolation by insertional mutagenesis.
Overexpression of the Activator (Ac) transposase gene in Arabidopsis thaliana resulted in a minimal germinal transposition frequency of 27% in which independent Dissociation (Ds) transposition eventsExpand
Supercoiled circular DNA in crown-gall inducing Agrobacterium strains.
By alkaline sucrose gradient, neutral sucrose gradient and dye-buoyant density centrifugation a large plasmid was shown to be present in the crown-gall inducing Agrobacterium tumefaciens strainExpand