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Bone marrow cells produce a novel TSHβ splice variant that is upregulated in the thyroid following systemic virus infection
Although cells of the immune system can produce thyroid-stimulating hormone (TSH), the significance of that remains unclear. Using 5′ rapid amplification of cDNA ends (RACE), we show that mouse boneExpand
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Genome wide interactions of wild-type and activator bypass forms of σ54
Enhancer-dependent transcription involving the promoter specificity factor σ54 is widely distributed amongst bacteria and commonly associated with cell envelope function. For transcriptionExpand
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Cellular and molecular phenotypes depending upon the RNA repair system RtcAB of Escherichia coli
RNA ligases function pervasively across the three kingdoms of life for RNA repair, splicing and can be stress induced. The RtcB protein (also HSPC117, C22orf28, FAAP and D10Wsu52e) is one suchExpand
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Single-step method for β-galactosidase assays in Escherichia coli using a 96-well microplate reader
Historically, the lacZ gene is one of the most universally used reporters of gene expression in molecular biology. Its activity can be quantified using an artificial substrate,Expand
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Mutations in RNA Polymerase Bridge Helix and Switch Regions Affect Active-Site Networks and Transcript-Assisted Hydrolysis
In bacterial RNA polymerase (RNAP), the bridge helix and switch regions form an intricate network with the catalytic active centre and the main channel. These interactions are important forExpand
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A data comparison between a traditional and the single-step β-galactosidase assay
This article describes reproducibility of a single-step automated β-galactosidase, and the equivalence of its data to the traditional assay (“Experiments in Molecular Genetics” [1]). This was doneExpand
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A CRISPR-associated Rossmann fold (CARF) domain regulates transcription of an RNA repair system in Escherichia coli
CRISPR-associated Rossmann fold (CARF) domain signalling underpins the modulation of CRISPR-Cas systems. Unlike the majority of known CARF domains associated with nuclease activity in CRISPR-CasExpand
The acute myeloid leukaemia market
most common types of leukaemia in adults; ~21,000 new cases are expected in the United States alone in 2019. The median age of diagnosis is 68 years and the 5-year overall survival (OS) is 28%. AMLExpand