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Activation of thiamin diphosphate in enzymes.
TLDR
The data of the thiamin diphosphate analogues and of the mutant enzymes show the N1' atom and the 4'-NH2 group to be essential for the activation of the coenzyme and a conserved glutamate involved in the proton abstraction mechanism of the enzyme-boundThDP. Expand
Cosecretion of Chaperones and Low-Molecular-Size Medium Additives Increases the Yield of Recombinant Disulfide-Bridged Proteins
TLDR
Low-molecular-size additive l-arginine and the redox compound glutathione were added to the culture medium and the effects of cosecreted ATP-independent molecular chaperones on the yields of native therapeutic proteins were investigated. Expand
Purification process of G-CSF
Purification process of recombinant granulocyte colony stimulating (G-CSF), comprising at least two cation exchange chromatographies which are respectively performed before and after the hydrophobicExpand
[Analysis of iomeglamic acid (Falignost)].
Purification procedure GCSF.
Purification process of recombinant colony stimulating granulocyte (G-CSF), comprising at least one cation exchange chromatography and at least one hydrophobic interaction chromatography factor,Expand
[Biotransformation of iomeglamic acid (Falignost)].
Activation of thiamin diphosphate in enzymes 1 BIOCHIMICA
2 . M a t e r i a l s a n d m e t h o d s . 2 . 1 . P r o t e i n p r e p a r a t i o n . 2 . 2 . C o e n z y m e a n a l o g u e s . 2 . 3 . N M R e x p e r i m e n t s . 2 2 2 2 2 2 2 2 3 2 2 3 A bExpand