• Publications
  • Influence
A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote Chlamydomonas
TLDR
It is shown here that the unicellular alga Chlamydomonas does indeed use a rhodopsin photoreceptor, and that 11-cis-retinal is the natural chromophore and that the protein environment of this retinal is similar to that found in bovine rhodopin, suggesting homology with the r Rhodopsins of higher organisms. Expand
Rhodopsin guides fungal phototaxis
Activation of Chlamydomonas rhodopsin in vivo does not require isomerization of retinal.
TLDR
The unicellular eukaryote Chlamydomonas reinhardtii is a phototactic alga that swims toward or away from light, using rhodopsin as the photopigment, and an attractive hypothesis is that charge redistribution in the excited state of retinal directly triggers the activity of r Rhodopsin. Expand
An electro-optic monitor of the behavior of Chlamydomonas reinhardtii cilia.
TLDR
How to record ciliary motion continuously for long periods is described and a response that is linear with absolute light intensity as well as different kinds of response that are clearly nonlinear are seen, implying two signaling pathways from the cell body to the cilia. Expand
Spectral sensitivity, structure and activation of eukaryotic rhodopsins: activation spectroscopy of rhodopsin analogs in Chlamydomonas.
TLDR
A unique structural model for eukaryotic rhodopsins is defined and the spectral clustering of pigments, the spectral differences between red and green rhodopins and the molecular basis of color blindness are explained. Expand
Linear systems analysis of the ciliary steering behavior associated with negative-phototaxis in Chlamydomonas reinhardtii.
TLDR
The use of two different approaches that enable the trans cilium to respond ahead of the cis for both the beating frequency and stroke velocity responses suggests the importance of both responses to phototaxis. Expand
Evidence from Chlamydomonas on the photoactivation of rhodopsins without isomerization of their chromophore.
TLDR
Responses to chromophores formed from naphthalene aldehydes suggest that charge separation within the excited chromophore leads to electric field-induced polarization of nearby amino acid residues and altered hydrogen bonding, which facilitates the reported multiple bond rotations and protein rearrangements of rhodopsin activation. Expand
ALL‐trans‐RETINAL IS THE CHROMOPHORE BOUND TO THE PHOTORECEPTOR OF THE ALGA: Chlamydomonas reinhardtii *
TLDR
All‐trans‐retinal is the native molecule that is available to be incorporated into the rhodopsin of Chlamydomonas and therefore forms the functioning chromophore on binding, and is identified as the existing retinaloxime isomer. Expand
Autoregulation of rhodopsin synthesis in Chlamydomonas reinhardtii.
TLDR
The time lag required for induction of retinal synthesis and preliminary experiments with transcription or translation inhibitors suggest that alterations in gene expression could be involved in the induction process, which could be similar to other processes in which membrane receptors for hormones, neurotransmitters, or growth factors regulate gene expression. Expand
...
1
2
3
...