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Most of centrin in animal cells is not centrosome-associated and centrosomal centrin is confined to the distal lumen of centrioles.
Centrin is a member of the calcium-binding EF-hand protein superfamily present in centrosomes of widely divergent species. Investigating the cellular distribution of human centrin by bothExpand
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Centrin plays an essential role in microtubule severing during flagellar excision in Chlamydomonas reinhardtii
Previously, we reported that flagellar excision in Chlamydomonas reinhardtii is mediated by an active process whereby microtubules are severed at select sites within the flagellar-basal bodyExpand
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Cloning of a cDNA encoding human centrin, an EF-hand protein of centrosomes and mitotic spindle poles.
A human cDNA expression library was screened using anti-centrin antibodies to obtain a cDNA clone encoding human centrin. The cDNA clone contains an open reading frame of 516 base pairs and predictsExpand
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Actin dynamics during the cell cycle in Chlamydomonas reinhardtii.
We have used two monoclonal antibodies to demonstrate the presence and localization of actin in interphase and mitotic vegetative cells of the green alga Chlamydomonas reinhardtii. CommerciallyExpand
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Altered centrosome structure is associated with abnormal mitoses in human breast tumors.
Centrosomes are the major microtubule organizing center in mammalian cells and establish the spindle poles during mitosis. Centrosome defects have been implicated in disease and tumor progression andExpand
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Centrin-mediated microtubule severing during flagellar excision in Chlamydomonas reinhardtii
Chlamydomonas cells excise their flagella in response to a variety of experimental conditions (e.g., extremes of temperature or pH, alcohol or detergent treatment, and mechanical shear). Here, weExpand
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Striated flagellar roots: isolation and partial characterization of a calcium-modulated contractile organelle
We report the isolation of striated flagellar roots from the Prasinophycean green alga Tetraselmis striata using sedimentation in gradients of sucrose and flotation on gradients of colloidal silica.Expand
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The centrin-based cytoskeleton of Chlamydomonas reinhardtii: distribution in interphase and mitotic cells
Monoclonal and polyclonal antibodies raised against algal centrin, a protein of algal striated flagellar roots, were used to characterize the occurrence and distribution of this protein in interphaseExpand
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CP110 cooperates with two calcium-binding proteins to regulate cytokinesis and genome stability.
The centrosome is an integral component of the eukaryotic cell cycle machinery, yet very few centrosomal proteins have been fully characterized to date. We have undertaken a series of biochemical andExpand
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Role of coated vesicles, microfilaments, and calmodulin in receptor- mediated endocytosis by cultured B lymphoblastoid cells
Cell surface receptor IgM molecules of cultured human lymlphoblastoid cells (WiL2) patch and redistribute into a cap over the Golgi region of the cell after treatment with multivalent anti-IgMExpand
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