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Structure of the outer membrane translocator domain of the Haemophilus influenzae Hia trimeric autotransporter
The crystal structure of the C‐terminal end of Hia, corresponding to the entire Hia translocator domain and part of the passenger domain, is presented, providing important insights into the mechanism of translocation in trimeric autotransporters. Expand
Trimeric autotransporters: a distinct subfamily of autotransporter proteins.
It is speculated that trimeric autotransporters evolved to enable high-affinity multivalent adhesive interactions with host surfaces and circulating host molecules to take place. Expand
Prevalence and distribution of the hmw and hia genes and the HMW and Hia adhesins among genetically diverse strains of nontypeable Haemophilus influenzae.
The prevalence and distribution of the hmw and hia genes in a collection of 59 nontypeable strains previously characterized in terms of genetic relatedness were determined and it was established that the IS1016 insertion element was uniformly absent from strains containing hmW sequences but was present in two-thirds of thehmw-deficient strains. Expand
Structural basis for host recognition by the Haemophilus influenzae Hia autotransporter
The crystal structure of the high‐affinity HiaBD1 binding domain is described, which has a novel trimeric architecture with three‐fold symmetry and a mushroom shape, and provides potential for a multivalent interaction with the host cell surface. Expand
Molecular determinants of the pathogenesis of disease due to non-typable Haemophilus influenzae.
The present understanding of the molecular determinants of this sequence of events of non-typable H. influenzae pathogenicity should facilitate development of novel approaches to the treatment and prevention of H. Influenzae disease. Expand
Induction of Proinflammatory Cytokines from Human Respiratory Epithelial Cells after Stimulation by NontypeableHaemophilus influenzae
It is suggested that bacterial molecules beyond previously described NTHi cell surface adhesins and LOS play a role in the induction of proinflammatory cytokines from respiratory epithelial cells. Expand
Kingella kingae: An Emerging Pathogen in Young Children
Kingella kingae is being recognized increasingly as a common etiology of pediatric osteoarticular infections, bacteremia, and endocarditis, which reflects improved culture methods and use of nucleicExpand
The Haemophilus influenzae HMW1 adhesin is glycosylated in a process that requires HMW1C and phosphoglucomutase, an enzyme involved in lipooligosaccharide biosynthesis
It is concluded that glycosylation is a prerequisite for H MW1 stability and appears to be essential for optimal HMW1 tethering to the bacterial surface, which in turn is required for HMW 1‐mediated adherence, thus revealing a novel mechanism by which gly cosylation influences cell–cell interactions. Expand
Kingella kingae Expresses Type IV Pili That Mediate Adherence to Respiratory Epithelial and Synovial Cells
It is found that type IV pili and the two PilC homologs play important roles in mediating K. kingae adherence and that the number of pili expressed by the bacterium correlates with the level of adherence to synovial cells but not to respiratory cells. Expand
A Haemophilus influenzae IgA protease‐like protein promotes intimate interaction with human epithelial cells
It is speculated that the hap gene product is an important determinant of colonization, perhaps enabling the organism to evade the local immune response and thereby persist within the respiratory tract. Expand