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The biosynthetic gene cluster for the antitumor rebeccamycin: characterization and generation of indolocarbazole derivatives.
The cloned genes should help to elucidate the molecular basis for indolocarbazole biosynthesis and set the stage for the generation of novel indolo-rebeccamycin analogues by genetic engineering.
Angucyclines: Biosynthesis, mode-of-action, new natural products, and synthesis.
The main focus of this article is on new synthetic approaches and biosynthetic investigations, most of which were published between 1997 and 2010, but go beyond, e.g. for some biosyntheses all the way back to the 1980s, to provide the necessary context of information.
An audience response system strategy to improve student motivation, attention, and feedback.
Focused, strategically placed ARS questions throughout lectures may help students maintain attention and stay motivated to learn and allows instructors to adapt lectures to address areas of deficiency.
Angucycline group antibiotics.
Combinatorial biosynthesis of antitumor indolocarbazole compounds.
A biological process based on combinatorial biosynthesis for the production of indolocarbazole compounds (or their precursors) in engineered microorganisms as a complementary approach to chemical synthesis is reported.
Mithramycin SK, a novel antitumor drug with improved therapeutic index, mithramycin SA, and demycarosyl-mithramycin SK: three new products generated in the mithramycin producer Streptomyces
The structures of these three compounds confirmed indirectly the proposed role of MtmW in MTM biosynthesis, and the new mithramycin derivatives bear unexpectedly shorter 3-side chains than MTM, presumably caused by nonenzymatic rearrangement or cleavage reactions of the initially formed pentyl side chain with a reactive beta-dicarbonyl functional group.
Modification of post-PKS tailoring steps through combinatorial biosynthesis.
This review covers the highlights of combinatorial biosynthesis applied on post-polyketide synthase modifying enzymes, such as oxygenases, and covers literature from 1985 to 2002.
Novel GC-rich DNA-binding compound produced by a genetically engineered mutant of the mithramycin producer Streptomyces argillaceus exhibits improved transcriptional repressor activity: implications
The new MTM derivative SDK, obtained by targeted gene inactivation of the ketoreductase MtmW catalyzing the last step in MTM biosynthesis, exhibited greater activity as transcriptional inhibitor compared to MTM and repressed transcription of multiple genes implicated in critical aspects of cancer development and progression.
The complete gene cluster of the antitumor agent gilvocarcin V and its implication for the biosynthesis of the gilvocarcins.
This is the first reported gene cluster encoding the biosynthesis of a benzo[d]naphtho[1,2-b]pyran-6-one aryl C-glycoside antibiotic, which lays the foundation for the detailed studies of its intriguing biosynthetic steps and possibly for the generation of gilvocarcin analogues with improved biological activities through combinatorial biosynthesis.
The dynamic structure of jadomycin B and the amino acid incorporation step of its biosynthesis.
Two findings led to the conclusion that a nonenzymatic reaction with the amino acid followed by a likewise nonenzysmatic cyclization cascade are crucial for its late biosynthesis of jadomycins.