Global Mapping of the Yeast Genetic Interaction Network
Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.
The establishment, inheritance, and function of silenced chromatin in Saccharomyces cerevisiae.
Current knowledge and models for how silenced chromatin in Saccharomyces cerevisiae forms, functions, and is inherited are described and emerging evidence that mutations that cause global changes in the modification of histones can alter the balance between euchromatin and silenced Chromatin within a cell is described.
A Protein Complex Containing the Conserved Swi2/Snf2-Related ATPase Swr1p Deposits Histone Variant H2A.Z into Euchromatin
A novel role for a member of the ATP-dependent chromatin remodeling enzyme family is revealed in determining the region-specific histone subunit composition of chromatin in vivo and controlling the epigenetic state of Chromatin.
Four genes responsible for a position effect on expression from HML and HMR in Saccharomyces cerevisiae.
Evidence for the existence of four SIR genes is presented and some recessive mutations in different genes fail to complement in Saccharomyces cerevisiae, indicating unusual complementation properties for a number of sir mutations.
Role of 26S proteasome and HRD genes in the degradation of 3-hydroxy-3-methylglutaryl-CoA reductase, an integral endoplasmic reticulum membrane protein.
These data suggested a model of ER protein degradation in which the Hrd1p and Hrd3p proteins conspire to deliver HMG-R to the 26S proteasome, and lend in vivo support to the proposed role of the p97/TRAP-2/Hrd2p protein as a functionally important component of the 25S proteAsome.
Upc2p and Ecm22p, Dual Regulators of Sterol Biosynthesis in Saccharomyces cerevisiae
It is suggested that Saccharomyces cerevisiae and human cells regulate sterol synthesis by different mechanisms.
Ordered nucleation and spreading of silenced chromatin in Saccharomyces cerevisiae.
A model for the spreading of silenced chromatin involving cycles of nucleosome deacetylation by Sir2p followed by recruitment of additional Sir2 p, Sir3p, and Sir4p to the newly de acetylated nucleosomes is suggested and generalizable to other types of heritable chromatin states.
Modulation of Ras and a-Factor Function by Carboxyl-Terminal Proteolysis
Two genes in Saccharomyces cerevisiae, RCE1 and AFC1, were identified that appear to be responsible for the processing of prenylated proteins, which contain a covalently linked cholesterol intermediate near their carboxyl-termini.
Telomeric heterochromatin boundaries require NuA4-dependent acetylation of histone variant H2A.Z in Saccharomyces cerevisiae.
Results show a role for H2A.Z acetylation in restricting silent chromatin, and reveal that H2.Z and H4 can contribute to a common function essential to life.
Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae
GRFI and ABFI were both abundant DNA-binding factors and did not appear to be encoded by the SIR genes, whose products are required for repression of the silent mating type loci, indicating that both GRFI andABFI play multiple roles within the cell.