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Localization of the (1,3)β-D-glucan synthase catalytic subunit homologue Bgs1p/Cps1p from fission yeast suggests that it is involved in septation, polarized growth, mating, spore wall formation and
TLDR
The in vivo physiological localization of Bgs1p in a bgs1Δ strain containing a functional GFP-bgs1+ gene shows that it is localized to the areas of polarized cell wall growth and so it is proposed that it might be involved in synthesizing the lineal (1,3)β-D-glucan of the primary septum, as well as a similar lineals when other processes of cell wall grow or repair are needed.
The (1,3)β‐d‐glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation
TLDR
It is demonstrated that in the absence of Bgs1p abnormal septa are formed, but the cells cannot separate and eventually die, and linear (1,3)β‐d‐glucan is necessary but not sufficient for primary septum formation.
Extracellular cell wall β(1,3)glucan is required to couple septation to actomyosin ring contraction
β(1,3)glucan is critical for contractile ring positioning and for coupling septum synthesis to constriction of the contractile ring and plasma membrane extension during cytokinesis.
Decoying the cap- mRNA degradation system by a double-stranded RNA virus and poly(A)- mRNA surveillance by a yeast antiviral system
TLDR
It is shown that SKI2, SKI3, and SKI8 inhibit translation of electroporated luciferase and beta-glucuronidase mRNAs in vivo, but only if they lack the 3' poly(A) structure, suggesting a (nonessential) role in blocking translation of fragmented cellular m RNAs.
The novel fission yeast (1,3)β-D-glucan synthase catalytic subunit Bgs4p is essential during both cytokinesis and polarized growth
TLDR
Cloned bgs4+ and shown that Bgs4p is the only subunit found to be a part of the GS enzyme and essential for maintaining cell integrity during cytokinesis and polarized growth show for the first time an essential role for a Bgs subunit in the synthesis of a (1,3)β-D-glucan necessary to preserve cell integrity when cell wall synthesis or repair are needed.
Fission yeast Ags1 confers the essential septum strength needed for safe gradual cell abscission
The α(1-3)glucan synthase Ags1 is essential for both secondary septum formation and the primary septum structural strength needed to counter cell turgor pressure during cell separation.
Cooperation between Paxillin-like Protein Pxl1 and Glucan Synthase Bgs1 Is Essential for Actomyosin Ring Stability and Septum Formation in Fission Yeast
TLDR
It is indicated that CAR anchorage to the plasma membrane through Cdc15 and Pxl1, and concomitant Bgs1 activity, are necessary for CAR maintenance and septum formation in fission yeast.
Isolation and characterization of Saccharomyces cerevisiae mutants resistant to Calcofluor white
TLDR
Cell wall analysis evidenced a five- to ninefold reduction in the amount of chitin in mutants compared with that in the wild-type strain; however, the percentage of beta-glucan that remained insoluble after alkali extraction was considerably reduced in mutant cells.
cps1+, a Schizosaccharomyces pombe gene homolog of Saccharomyces cerevisiae FKS genes whose mutation confers hypersensitivity to cyclosporin A and papulacandin B
TLDR
A DNA fragment containing the cps1+ gene was cloned, and sequence analysis indicated that it encodes a predicted membrane protein of 1,729 amino acids with 15 to 16 transmembrane domains that might be a catalytic or an associated copurifying subunit of the fission yeast 1,3-beta-D-glucan synthase that plays an essential role in cell wall synthesis.
Pol of gag–pol fusion protein required for encapsidation of viral RNA of yeast L-A virus
TLDR
It is shown that Gag alone is sufficient for virus particle formation, but that it fails to package the viral single-stranded RNA genome, consistent with the L-A virus encapsidation model.
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