J. Ramos

Publications491
h-index82
Citations24,137
Highly Influential Citations1,242
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The TetR Family of Transcriptional Repressors
TLDR
A general profile for the proteins of the TetR family of repressors is developed, made up of 47 amino acid residues that correspond to the helix-turn-helix DNA binding motif and adjacent regions in the three- dimensional structures of TetR, QacR, CprB, and EthR, four family members for which the function and three-dimensional structure are known.
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Arac/XylS family of transcriptional regulators.
TLDR
The phylogenetic relationship suggests that members of the AraC/XylS family have recruited the nonconserved domain(s) into a series of existing domains involved in DNA recognition and transcription stimulation and that this recruited domain governs the role that the regulator carries out.
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Biological Degradation of 2,4,6-Trinitrotoluene
TLDR
A number of biotechnological applications of bacteria and fungi, including slurry reactors, composting, and land farming, to remove TNT from polluted soils are discussed, which are highly efficient in removing TNT.
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Mechanisms of solvent tolerance in gram-negative bacteria.
TLDR
The number of efflux pump operons has been found to correlate with the degree of solvent tolerance in different P. putida strains, and the operation of these efflux pumps seems to be coupled to the proton motive force via the TonB system, although the intimate mechanism of energy transfer remains elusive.
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Transition from reversible to irreversible attachment during biofilm formation by Pseudomonas fluorescens WCS365 requires an ABC transporter and a large secreted protein
TLDR
The identification of an ATP‐binding cassette (ABC) transporter and an associated large cell‐surface protein that are required for biofilm formation by Pseudomonas fluorescens WCS365 are reported and it is proposed that the lapEBC‐encoded ABC transporter participates in the secretion of LapA.
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Regulator and enzyme specificities of the TOL plasmid-encoded upper pathway for degradation of aromatic hydrocarbons and expansion of the substrate range of the pathway
TLDR
Toluene oxidase acts as the bottleneck step for the catabolism of p-ethyl- and m- and p-chlorotoluene through the TOL upper pathway.
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Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators.
The xyl genes of Pseudomonas putida TOL plasmid that specify catabolism of toluene and xylenes are organized in four transcriptional units: the upper-operon xylUWCAMBN for conversion of…
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Efflux Pumps Involved in Toluene Tolerance in Pseudomonas putida DOT-T1E
TLDR
The basic mechanisms underlying solvent tolerance in Pseudomonas putida DOT-T1E are efflux pumps that remove the solvent from bacterial cell membranes and the mutant was unable to remove 1,2,4-[14C]trichlorobenzene from the cell membranes when grown on Luria-Bertani medium but was able to remove the aromatic compound when pregrown on LB medium with toluene supplied via the gas phase.
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Comparative genomics and functional analysis of niche-specific adaptation in Pseudomonas putida
TLDR
Comparative genomics provided a powerful tool to gain new insights into the adaptation of P. putida to specific lifestyles and environmental niches, and clearly demonstrated that horizontal gene transfer played a key role in this adaptation process.
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