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Hydrophobic interaction chromatography of proteins.
In this article, an overview of hydrophobic interaction chromatography (HIC) of proteins is given. After a brief description of protein hydrophobicity and hydrophobic interactions, we present theExpand
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Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA.
The diversity of biomolecules present in plasmid DNA (pDNA)-containing extracts and the structural and chemical similarities between pDNA and impurities are some of the main challenges of improvingExpand
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Chromatography of plasmid DNA.
Liquid chromatography plays a central role in process-scale manufacturing of therapeutic plasmid DNA (pDNA) for gene therapy and DNA vaccination. Apart from its use as a preparative purificationExpand
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Advances in chromatographic supports for pharmaceutical-grade plasmid DNA purification.
Chromatographic technology is undoubtedly one of the most diverse and powerful purification methods for downstream process applications. Expand
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Pichia pastoris: a recombinant microfactory for antibodies and human membrane proteins.
During the last few decades, it has become evident that the compatibility of the yeast biochemical environment with the ability to process and translate the RNA transcript, along with its capacity toExpand
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Biomedical application of plasmid DNA in gene therapy: A new challenge for chromatography
Abstract Gene therapy and DNA vaccination are clinical fields gradually emerging in the last few decades, in particular after the discovery of some gene-related diseases. The increased relevance ofExpand
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Nanoparticle mediated delivery of pure P53 supercoiled plasmid DNA for gene therapy.
The translation of non-viral gene replacement therapies for cancer into clinical application is currently hindered due to known issues associated with the effectiveness of plasmid DNA (pDNA)Expand
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Rhodamine based plasmid DNA nanoparticles for mitochondrial gene therapy.
Conventional treatments for patients suffering from mitochondrial cytopathies are, in most of the cases, inefficient and there is, until now, no effective cure. Mitochondrial gene therapy can be seenExpand
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Advances in time course extracellular production of human pre-miR-29b from Rhodovulum sulfidophilum
The present study reports the successful production of human pre-miR-29b both intra- and extracellularly in the marine phototrophic bacterium Rhodovulum sulfidophilum using recombinant RNAExpand
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A novel prokaryotic expression system for biosynthesis of recombinant human membrane-bound catechol-O-methyltransferase.
Membrane proteins constitute 20-30% of all proteins encoded by the genome of various organisms. While large amounts of purified proteins are required for pharmaceutical and crystallization attempts,Expand
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