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Fluorescence In Situ Hybridization and Catalyzed Reporter Deposition for the Identification of Marine Bacteria
ABSTRACT Fluorescence in situ hybridization (FISH) with horseradish peroxidase (HRP)-labeled oligonucleotide probes and tyramide signal amplification, also known as catalyzed reporter depositionExpand
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Contribution of Archaea to Total Prokaryotic Production in the Deep Atlantic Ocean
ABSTRACT Fluorescence in situ hybridization (FISH) in combination with polynucleotide probes revealed that the two major groups of planktonic Archaea (Crenarchaeota and Euryarchaeota) exhibit aExpand
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Comparative 16S rRNA Analysis of Lake Bacterioplankton Reveals Globally Distributed Phylogenetic Clusters Including an Abundant Group of Actinobacteria
ABSTRACT In a search for cosmopolitan phylogenetic clusters of freshwater bacteria, we recovered a total of 190 full and partial 16S ribosomal DNA (rDNA) sequences from three different lakes (LakeExpand
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Culturability and In Situ Abundance of Pelagic Bacteria from the North Sea
ABSTRACT The culturability of abundant members of the domainBacteria in North Sea bacterioplankton was investigated by a combination of various cultivation strategies and cultivation-independent 16SExpand
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Predation on prokaryotes in the water column and its ecological implications
  • J. Pernthaler
  • Biology, Medicine
  • Nature Reviews Microbiology
  • 1 July 2005
The oxic realms of freshwater and marine environments are zones of high prokaryotic mortality. Lysis by viruses and predation by ciliated and flagellated protists result in the consumption ofExpand
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Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes
Publisher Summary The chapter discusses the fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes, and describes probe designing and testing. Fluorescence FISH withExpand
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Combining Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization and Microautoradiography To Detect Substrate Utilization by Bacteria and Archaea in the Deep Ocean
ABSTRACT The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resultedExpand
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Succession of Pelagic Marine Bacteria during Enrichment: a Close Look at Cultivation-Induced Shifts
ABSTRACT Enrichment experiments with North Sea bacterioplankton were performed to test if rapid incubation-induced changes in community structure explain the frequent isolation of members of a fewExpand
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An Improved Protocol for Quantification of Freshwater Actinobacteria by Fluorescence In Situ Hybridization
ABSTRACT We tested a previously described protocol for fluorescence in situ hybridization of marine bacterioplankton with horseradish peroxidase-labeled rRNA-targeted oligonucleotide probes andExpand
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Seasonality in bacterial diversity in north-west Mediterranean coastal waters: assessment through clone libraries, fingerprinting and FISH.
We combined denaturing gradient gel electrophoresis (DGGE), catalysed reporter deposition-FISH (CARD-FISH) and clone libraries to investigate the seasonality of the bacterial assemblage compositionExpand
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