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Handbook of Biological Confocal Microscopy
Foundations of Confocal Scanned Imaging in Light Microscopy -- Fundamental Limits in Confocal Microscopy -- Special Optical Elements -- Points, Pixels, and Gray Levels: Digitizing Image Data -- LaserExpand
Fundamental Limits in Confocal Microscopy
The previous chapter described how the confocal approach developed from conventional light microscopy and outlined the basic advantages gained by the use of confocal sampling techniques, primarilyExpand
Points, Pixels, and Gray Levels: Digitizing Image Data
TLDR
Microscopical images are now almost always recorded digitally, and the flux of photons that forms the final image must be divided into small geometrical subunits called pixels, which will be stored as a single number. Expand
Confocal and two‐photon microscopy: Foundations, applications and advances
Description: Confocal and Two–Photon Microscopy Foundations, Applications, and Advances Edited by Alberto Diaspro Confocal and two–photon fluorescence microscopy has provided researchers with uniqueExpand
Low voltage scanning electron microscopy
  • J. Pawley
  • Chemistry, Medicine
  • Journal of microscopy
  • 1 October 1984
TLDR
The advantages of low voltage operation, recent progress in instrumentation and a prototype instrument designed and built for optimum performance at 1 kV are described, which substantially removes the practical deterrents to operation in this mode. Expand
The development of field-emission scanning electron microscopy for imaging biological surfaces.
  • J. Pawley
  • Chemistry, Medicine
  • Scanning
  • 1 August 1997
This article traces the important milestones in the development of high-resolution, field-emission, scanning electron microscopes (SEM). Such instruments are now capable of producing images of theExpand
Handbook of Biological Confocal Microscopy, Third Edition
This PDF file contains the editorial “Handbook of Biological Confocal Microscopy, Third Edition” for JBO Vol. 13 Issue 02
Wave of free calcium at fertilization in the sea urchin egg visualized with fura-2.
TLDR
A wave front of increased free calcium traversing the egg at fertilization is demonstrated in the sea urchin Lytechinus pictus and two waves of Ca2+ originating from the respective points of sperm entry are recognized. Expand
Disk-Scanning Confocal Microscopy
Rapid biological imaging of faint fluorophores in living cells — especially in four dimensions [three dimensions + time] — imposes different instrumentation challenges from slowly acquiring a singleExpand
High-resolution scanning electron microscopy.
TLDR
With state-of-the-art electron optics, resolutions of the order of 1 nm are now possible and progress beyond this level may be restricted by the delocalization of SE production and ultimate limits to electron-optical performance. Expand
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