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Establishment of Centromeric Chromatin by the CENP-A Assembly Factor CAL1 Requires FACT-Mediated Transcription.
TLDR
It is demonstrated that CENP-A assembly by its loading factor, CAL1, requires RNAPII-mediated transcription of the underlying DNA, which establishes RNAP II passage as a key step in chaperone-mediated CENp-A chromatin establishment and propagation. Expand
Islands of retroelements are major components of Drosophila centromeres
TLDR
Contrary to previous models that heralded satellite repeats as the major functional components, it is demonstrated that functional centromeres form on islands of complex DNA sequences enriched in retroelements that are flanked by large arrays of satellite repeats. Expand
Broad geographic analyses reveal varying patterns of genetic diversity and host specificity among echinostome trematodes in New Zealand snails
TLDR
The results suggest that host specificity is determined by host–parasite incompatibilities, not geographic separation, and that it can evolve in different ways in closely related parasite lineages. Expand
Construction of adenoviral vectors.
TLDR
A negative selection based on the traditional cotransfection method using viral DNA from an E1-deleted adenoviral recombinant that expresses green fluorescent protein (GFP), which allows for the detection of contaminating parental virus at later stages when production lots of the recombinant vector are being made. Expand
Construction of adenoviral vectors
TLDR
A negative selection based on the traditional cotransfection method using viral DNA from an E1-deleted adenoviral recombinant that expresses green fluorescent protein (GFP), which allows for the detection of contaminating parental virus at later stages when production lots of the recombinant vector are being made. Expand
Islands of retroelements are the major components of Drosophila centromeres
TLDR
Contrary to previous models that heralded satellite repeats as the major functional components, it is demonstrated that functional centromeres form on islands of complex DNA sequences enriched in retroelements that are flanked by large arrays of satellite repeats. Expand
Targeted De Novo Centromere Formation in Drosophila Reveals Plasticity and Maintenance Potential of CENP-A Chromatin.
TLDR
The results underpin the ability of CENP-A chromatin to establish and sustain mitotic centromere function in Drosophila and show that these centromeres can be maintained through development. Expand
The KAT's Out of the Bag: Histone Acetylation Promotes Centromere Assembly.
TLDR
It is revealed that a critical role of the Mis18 complex is to transiently recruit the lysine acetyltransferase KAT7 to centromeres to facilitate the removal of H3K9me3 and the deposition of CENP-A. Expand