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Mechanical Devices of the Spliceosome: Motors, Clocks, Springs, and Things
We thank J. Thorner, J. Lorsch, and D. Herschlag for provocative discussions; J. Abelson, R. Luhrmann, T. Nilsen, and R. Reed for preprints; and G. Chanfreau, C. Collins, A. Frankel, A. Kistler, K.Expand
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The EF-G-like GTPase Snu114p regulates spliceosome dynamics mediated by Brr2p, a DExD/H box ATPase.
Binding of a pre-mRNA substrate triggers spliceosome activation, whereas the release of the mRNA product triggers spliceosome disassembly. The mechanisms that underlie the regulation of theseExpand
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An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28p.
Pre-mRNA splicing requires dramatic RNA rearrangements hypothesized to be catalyzed by ATP-dependent RNA unwindases of the DExD/H box family. In a rearrangement critical for the fidelity of 5' spliceExpand
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Exon ligation is proofread by the DExD/H-box ATPase Prp22p
To produce messenger RNA, the spliceosome excises introns from precursor (pre)-mRNA and splices the flanking exons. To establish fidelity, the spliceosome discriminates against aberrant introns, butExpand
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Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD box splicing factor.
While some members of the ubiquitous DExD/H box family of proteins have RNA helicase activity in vitro, their roles in vivo remain virtually unknown. Here, we show that the function of an otherwiseExpand
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U2 toggles iteratively between the stem IIa and stem IIc conformations to promote pre-mRNA splicing.
To ligate exons in pre-messenger RNA (pre-mRNA) splicing, the spliceosome must reposition the substrate after cleaving the 5' splice site. Because spliceosomal small nuclear RNAs (snRNAs) bind theExpand
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Autosomal-dominant retinitis pigmentosa caused by a mutation in SNRNP200, a gene required for unwinding of U4/U6 snRNAs.
Mutations in genes associated with the U4/U6-U5 small nuclear ribonucleoprotein (snRNP) complex of the spliceosome are implicated in autosomal-dominant retinitis pigmentosa (adRP), a group ofExpand
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A role for ubiquitin in the spliceosome assembly pathway
The spliceosome uses numerous strategies to regulate its function in mRNA maturation. Ubiquitin regulates many cellular processes, but its potential roles during splicing are unknown. We haveExpand
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The Splicing Factor Prp43p, a DEAH Box ATPase, Functions in Ribosome Biogenesis
ABSTRACT Biogenesis of the small and large ribosomal subunits requires modification, processing, and folding of pre-rRNA to yield mature rRNA. Here, we report that efficient biogenesis of both small-Expand
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Intronic sequence elements impede exon ligation and trigger a discard pathway that yields functional telomerase RNA in fission yeast.
The fission yeast telomerase RNA (TER1) precursor harbors an intron immediately downstream from its mature 3' end. Unlike most introns, which are removed from precursor RNAs by the spliceosome in twoExpand
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