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A freeze-squeeze method for recovering long DNA from agarose gels.
Long DNA can be recovered from agarose gels after electrophoresis by freezing the gel slices and manually squeezing out liquid containing the DNA. With this method the recoveries of phage T7 DNAExpand
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Genetic complexity of mitochondrial DNA from Euglena gracilis.
Abstract We have determined the genetic complexity of mtDNA from Euglena gracilis, var. bacillaris, by DNA renaturation experiments. Renaturation was followed by the specific digestion ofExpand
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Fine structure of the 21S ribosomal RNA region on yeast mitochondria DNA. I. Construction of the physical map and localization of the cistron for the 21S mitochondrial ribosomal RNA.
1. We have used restriction enzyme analysis of petite mtDNAs to construct a detailed physical map of the 21S region on the mtDNA of the Saccharomyces cerevisiae strain JS1-3D. The map covers aExpand
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Nature of the base sequence conserved in the mitochondrial DNA of a low-density petite.
Abstract 1. 1. We have previously shown that the mtDNA of the ethidium-induced cytoplasmic petite mutant RD1A of the yeast Saccharomyces cerevisiae consists of perfect tandem repetitions of a veryExpand
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Sequence homology of nuclear and mitochondrial DNAs of different yeasts.
1. Both nuclear and mtDNA of four different yeasts show approximately 10% homology as measured by DNA-DNA filter hybridization. These homologous sequences are mainly attributable to the ribosomalExpand
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Properties of mitochondrial DNA from Kluyveromyces lactis.
Abstract 1. We have isolated a closed circular DNA fraction from mitochondria purified from Kluyveromyces lactis by centrifuging a mitochondrial lysate to equilibrium in CsCl containing ethidiumExpand
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Biochemical methods to locate genes on the physical map of yeast mitochondrial DNA.
Publisher Summary This chapter discusses biochemical methods to locate genes on the physical map of yeast mitochondrial DNA. The mtDNA of the yeasts Saccharomyces carlsbergensis and SaccharomycesExpand
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