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Nuclear Transfer Protocol Affects Messenger RNA Expression Patterns in Cloned Bovine Blastocysts
An aberrant expression pattern in NT embryos was found with respect to genes thought to be involved in stress adaptation, trophoblastic function, and DNA methylation during preimplantation development.
Cloned Cattle Fetuses with the Same Nuclear Genetics Are More Variable Than Contemporary Half-Siblings Resulting from Artificial Insemination and Exhibit Fetal and Placental Growth Deregulation Even…
In vitro culture and incomplete reprogramming of the donor genome are epigenetic effects that may override genetic traits and contribute to the greater variability in placental and fetal development in the NT group compared with AI half-siblings.
Coordination between donor cell type and cell cycle stage improves nuclear cloning efficiency in cattle.
Cloned cattle derived from a novel zona-free embryo reconstruction system.
A novel zona-free cloning method is described that doubles the throughput in cloned bovine embryo production over current procedures and generates viable offspring with the same efficiency.
Nuclear factors interact with conserved A/T-rich elements upstream of a nodule-enhanced glutamine synthetase gene from French bean.
The data suggest that PNF-1 and PRF-1 belong to an evolutionarily conserved group of nuclear factors that interact with specific A/T-rich sequences in a diverse set of plant genes.
Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris
The isolation and nucleotide sequencing of two essentially full‐length GS cDNA clones from a root cDNA library and the deduced amino acid sequences of the corresponding GS subunits are reported, indicating that the R‐2 gene is specifically repressed during nodule development.
Effect of antifreeze proteins on the motility of ram spermatozoa.
Addition of AFP or AFGP to the freezing medium at concentrations of 0.1 to 10 micrograms/ml significantly reduced the loss in spermatozoal motility that occurs due to the freeze-thaw process (P < 0.001).
Two glutamine synthetase genes from Phaseolus vulgaris L. display contrasting developmental and spatial patterns of expression in transgenic Lotus corniculatus plants.
It is concluded thatgln-beta and gln-gamma genes are differentially expressed both temporally and spatially in plant development and that the cis-acting regulatory elements responsible for conferring these contrasting expression patterns are located within a 2-kilobase region upstream of their coding sequences.
Motility of ram spermatozoa during storage in a chemically-defined diluent containing antioxidants.