• Publications
  • Influence
A novel methodology employing Corynebacterium glutamicum genome information to generate a new L-lysine-producing mutant
Abstract. Classical whole-cell mutagenesis has achieved great success in development of many industrial fermentation strains, but has the serious disadvantage of accumulation of uncharacterizedExpand
  • 268
  • 15
  • PDF
A novel gnd mutation leading to increased L-lysine production in Corynebacterium glutamicum.
Toward more efficient L-lysine production, we have been challenging genome-based strain breeding by the approach of assembling only relevant mutations in a single wild-type background. Following theExpand
  • 117
  • 8
A genome-based approach to create a minimally mutated Corynebacterium glutamicum strain for efficient l-lysine production
Based on the progress in genomics, we have developed a novel approach that employs genomic information to generate an efficient amino acid producer. A comparative genomic analysis of an industrialExpand
  • 111
  • 6
Anaerobic growth and potential for amino acid production by nitrate respiration in Corynebacterium glutamicum
Oxygen limitation is a crucial problem in amino acid fermentation by Corynebacterium glutamicum. Toward this subject, our study was initiated by analysis of the oxygen-requiring properties of C.Expand
  • 79
  • 6
A gene homologous to β-type carbonic anhydrase is essential for the growth of Corynebacterium glutamicum under atmospheric conditions
Abstract Carbonic anhydrase catalyzes the interconversion of CO2 and bicarbonate. We focused on this enzyme in the amino acid-producing organism Corynebacterium glutamicum in order to assess theExpand
  • 79
  • 5
Efficient 40°C fermentation of l-lysine by a new Corynebacterium glutamicum mutant developed by genome breeding
We have recently developed a new l-lysine-producing mutant of Corynebacterium glutamicum by "genome breeding" consisting of characterization and reconstitution of a mutation set essential forExpand
  • 76
  • 4
Large-scale production of GDP-fucose and Lewis X by bacterial coupling
A large-scale production system of GDP-fucose (GDP-Fuc) and fucosylated oligosaccharides was established by the combination of recombinant Escherichia coli cells overexpressing GDP-Fuc biosyntheticExpand
  • 52
  • 4
Transcriptome Analysis Reveals Global Expression Changes in an Industrial L-Lysine Producer of Corynebacterium glutamicum
Toward the elucidation of advanced mechanisms of L-lysine production by Corynebacterium glutamicum, a highly developed industrial strain B-6 was analyzed from the viewpoint of gene expression.Expand
  • 52
  • 3
Disruption of Malate:Quinone Oxidoreductase Increases L-Lysine Production by Corynebacterium glutamicum
Genomic analysis of a classically derived L-lysine-producing mutant, Corynebacterium glutamicum B-6, identified a nonsense mutation in the mqo gene, which encodes malate:quinone oxidoreductase (MQO).Expand
  • 22
  • 2
A leuC mutation leading to increased L-lysine production and rel-independent global expression changes in Corynebacterium glutamicum
We previously found by transcriptome analysis that global induction of amino acid biosynthetic genes occurs in a classically derived industrial l-lysine producer, Corynebacterium glutamicum B-6.Expand
  • 27
  • 1