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Cation binding and thermostability of FTHFS monovalent cation binding sites and thermostability of N10-formyltetrahydrofolate synthetase from Moorella thermoacetica.
Glu98 is a crucial residue in the interaction of monovalent cations with FTHFS, confirming that the removal of the unbalanced, buried charge of Glu98 stabilizes the enzyme.
Structural characterization of selenosubtilisin by selenium-77 NMR spectroscopy
The semisynthetic selenoenzyme selenosubtilisin catalyzes the reduction of alkyl hydroperoxides with concomitant oxidation of 3-carboxy-4-nitrobenzenethiol. To gain insight into the mechanism of this…
Purification and characterization of selenomethionyl thymidylate synthase from Escherichia coli: comparison with the wild-type enzyme.
- J. Boles, R. J. Cisneros, M. Weir, J. Odom, J. E. Villafranca, R. B. Dunlap
- Biology, ChemistryBiochemistry
- 19 November 1991
Comparisons of the properties of selenomethionyl thymidylate synthase isolated from Escherichia coli DL41 and wild-type (wt) enzyme obtained from E. coli Rue10 show no significant difference in conformation between the SeMet enzyme and the wt enzyme.
The crystal structure of N(10)-formyltetrahydrofolate synthetase from Moorella thermoacetica.
The structure was solved at 2.5 A resolution using multiwavelength anomalous dispersion (MAD) scattering by Se-Met residues. The subunit of N(10)-formyltetrahydrofolate synthetase is composed of…
Demonstration of the feasibility of observing nuclear magnetic resonance signals of 77Se covalently attached to proteins.
Selenium biochemistry chemical and physical studies
- J. Odom
A brief discussion of the vast and increasingly important area of organoselenium chemistry is presented as it relates to the biochemistry of this element. Organo selenoamino acids are the basic…
Increased lactate dehydrogenase isoenzyme 1 in serum and tumor tissue of a patient with small-cell carcinoma.
Histological examination of supraclavicular lymph node tissue obtained at biopsy from a 63-year-old man disclosed metastatic small-cell carcinoma showed persistently increased LD isoenzyme, with LD-1 greater than LD-2 pattern, emphasizing the importance of repetitive sampling for clinical interpretation of data on this enzyme.
MAGNETIC RESONANCE STUDIES OF SELENIUM CONTAINING COMPOUNDS
Abstract The utility of selenium-77 nuclear magnetic resonance in studying the properties of a range of selenium-containing compounds from small organoselenium molecules to macromolecules which…