• Publications
  • Influence
Pellet Formation and Fragmentation in Submerged Cultures of Penicillium chrysogenum and Its Relation to Penicillin Production
The spores of Penicillium chrysogenum are of the noncoagulating type, and after spore germination a culture of disperse mycelia is obtained. In this study, it is shown that when the hyphal elementsExpand
  • 117
  • 7
Hyphal growth and fragmentation of Penicillium chrysogenum in submerged cultures
A previously derived population model describing the average properties of hyphal elements in submerged cultures of filamentous fungi was revised, and a term for the influence fo spore germination onExpand
  • 84
  • 7
Metabolic engineering: techniques for analysis of targets for genetic manipulations.
  • J. Nielsen
  • Biology, Medicine
  • Biotechnology and bioengineering
  • 20 April 1998
Metabolic engineering has been defined as the purposeful modification of intermediary metabolism using recombinant DNA techniques. With this definition metabolic engineering includes: (1) insertingExpand
  • 153
  • 5
A Simple morphologically structured model describing the growth of filamentous microorganisms
  • J. Nielsen
  • Biology, Medicine
  • Biotechnology and bioengineering
  • 25 March 1993
Based on the reported mechanisms for filamentous growth, a simple morphologically structured growth model is set up. The model may describe the growth of filamentous microorganisms both on a solidExpand
  • 93
  • 5
Metabolic Control Analysis of the Penicillin Biosynthetic Pathway in a High‐Yielding Strain of Penicillium chrysogenum
Metabolic control analysis is used to identify the rate‐limiting step in the penicillin biosynthetic pathway in Penicillium chrysogenum. The analysis is carried out using a kinetic model for theExpand
  • 72
  • 5
Metabolic control analysis of biochemical pathways based on a thermokinetic description of reaction rates.
  • J. Nielsen
  • Chemistry, Medicine
  • The Biochemical journal
  • 1997
Metabolic control analysis is a powerful technique for the evaluation of flux control within biochemical pathways. Its foundation is the elasticity coefficients and the flux control coefficientsExpand
  • 66
  • 2
Transient disruption of non-homologous end-joining facilitates targeted genome manipulations in the filamentous fungus Aspergillus nidulans.
We have developed a transiently disrupted nkuA system in Aspergillus nidulans for efficient gene targeting. The nkuA disruption was made by inserting a counter-selectable marker flanked by a directExpand
  • 73
  • 2
Modelling the growth of filamentous fungi.
  • J. Nielsen
  • Biology, Medicine
  • Advances in biochemical engineering/biotechnology
  • 1992
Despite the considerable industrial importance of filamentous fungi there have been very few attempts to model the complex growth process of these microorganisms. With a new generation of highExpand
  • 64
  • 2
Modeling fermentations with recombinant microorganisms: Formulation of a structured model
A simple structured model is described and compared with experimental data for fermentations with recombinant. Escherichia coli. The model is a so‐called compartment, model, where the differentExpand
  • 48
  • 2
A new class of IMP dehydrogenase with a role in self-resistance of mycophenolic acid producing fungi
BackgroundMany secondary metabolites produced by filamentous fungi have potent biological activities, to which the producer organism must be resistant. An example of pharmaceutical interest isExpand
  • 32
  • 2
  • PDF