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Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related.
TLDR
Analysis of predicted amino acid sequences of these genes revealed strong conservation of both primary and secondary structure, suggesting that the particulate methane monooxygenase and ammonia mono Oxygenase are evolutionarily related enzymes despite their different physiological roles in these bacteria.
Stable-isotope probing as a tool in microbial ecology
TLDR
Stable-isotope probing offers a powerful new technique for identifying microorganisms that are actively involved in specific metabolic processes under conditions which approach those occurring in situ.
DNA stable-isotope probing
TLDR
General guidelines for incubating environmental samples with labeled substrate are highlighted and a detailed protocol for separating labeled DNA from unlabeled community DNA is provided, which maximizes the recovery oflabel DNA from CsCl gradients.
Metabolic aspects of aerobic obligate methanotrophy.
Improved group-specific PCR primers for denaturing gradient gel electrophoresis analysis of the genetic diversity of complex microbial communities
TLDR
Phylum- and class-specific PCR primers were tested for the production of clone libraries and for denaturing gradient gel electrophoresis (DGGE) analysis of complex bacterial communities, finding the application of the group-specific primers provides a higher resolution genetic fingerprinting approach than existing DGGE primer sets.
Molecular Ecology Techniques for the Study of Aerobic Methanotrophs
Methane oxidation can occur in both aerobic and anaerobic environments; however, these are completely different processes involving different groups of prokaryotes. Aerobic methane oxidation is
Characterization of Methanotrophic Bacterial Populations in Soils Showing Atmospheric Methane Uptake
TLDR
In this study the soil methane-oxidizing population was characterized by both labelling soil microbiota with14CH4 and analyzing a total soil monooxygenase gene library, and an unknown group of bacteria belonging to the α subclass of the class Proteobacteria was present.
Development and validation of a diagnostic microbial microarray for methanotrophs.
TLDR
A microarray targeting the particulate methane monooxygenase (pmoA) gene was developed for the detection and quantification of methanotrophs and functionally related bacteria and showed very good correlation with the expected results.
Stable isotope probing — linking microbial identity to function
TLDR
DNA and rRNA are the most informative taxonomic biomarkers and 13C-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation.
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