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Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related.
Genes encoding particulate methane monooxygenase and ammonia monooxygenase share high sequence identity. Degenerate oligonucleotide primers were designed, based on regions of shared amino acidExpand
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Stable-isotope probing as a tool in microbial ecology
Microorganisms are responsible for driving the biogeochemical cycling of elements on Earth. Despite their importance and vast diversity, the taxonomic identity of the microorganisms involved in anyExpand
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DNA stable-isotope probing
Stable-isotope probing is a method used in microbial ecology that provides a means by which specific functional groups of organisms that incorporate particular substrates are identified without theExpand
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Improved group-specific PCR primers for denaturing gradient gel electrophoresis analysis of the genetic diversity of complex microbial communities
Phylum- and class-specific PCR primers were tested for the production of clone libraries and for denaturing gradient gel electrophoresis (DGGE) analysis of complex bacterial communities. Primers wereExpand
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Metabolic aspects of aerobic obligate methanotrophy.
Publisher Summary This chapter discusses the metabolic aspects of aerobic obligate methanotrophy. Aerobic methanotrophs are a unique group of gram-negative bacteria that use methane as carbon andExpand
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Development and validation of a diagnostic microbial microarray for methanotrophs.
The potential of DNA microarray technology in high-throughput detection of bacteria and quantitative assessment of their community structures is widely acknowledged but has not been fully realisedExpand
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Molecular Ecology Techniques for the Study of Aerobic Methanotrophs
Methane oxidation can occur in both aerobic and anaerobic environments; however, these are completely different processes involving different groups of prokaryotes. Aerobic methane oxidation isExpand
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Stable-isotope probing of nucleic acids: a window to the function of uncultured microorganisms.
Phylogeny based on ribosomal RNA sequences alone is rarely a reliable indicator of microbial function. To circumvent this problem, nucleic acid based techniques have been developed that exploit theExpand
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Characterization of Methanotrophic Bacterial Populations in Soils Showing Atmospheric Methane Uptake
ABSTRACT The global methane cycle includes both terrestrial and atmospheric processes and may contribute to feedback regulation of the climate. Most oxic soils are a net sink for methane, and theseExpand
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Life without light: microbial diversity and evidence of sulfur- and ammonium-based chemolithotrophy in Movile Cave
Microbial diversity in Movile Cave (Romania) was studied using bacterial and archaeal 16S rRNA gene sequence and functional gene analyses, including ribulose-1,5-bisphosphate carboxylase/oxygenaseExpand
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