Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time Taqman PCR assay.
- D. Boyle, D. Boyle, V. Olsen, J. Morgan, A. Hyatt
- Biology, Environmental ScienceDiseases of Aquatic Organisms
- 9 August 2004
A real-time PCR Taqman assay that can accurately detect and quantify one zoospore in a diagnostic sample is developed that will assist the early detection of B. dendrobatidis in both captive and wild populations, thus facilitating treatment and protection of endangered populations, monitoring of pristine environments and preventing further global spread via amphibian trade.
Relative merits of nuclear ribosomal internal transcribed spacers and mitochondrial CO1 and ND1 genes for distinguishing among Echinostoma species (Trematoda)
Cryptic species, belonging to the 37 collar-spine Echinostoma group, were distinguished using nuclear rDNA ITS and mtDNA CO1 and ND1 sequences and it was indicated that ND1 is diverging significantly faster than the other 2 regions.
Nuclear rDNA ITS sequence variation in the trematode genus Echinostoma: an aid to establishing relationships within the 37-collar-spine group
DNA sequence data from the nuclear rDNA ITS1, 5·8S and ITS2 of 7 nominal species belonging to the 37-collar-spine group within the genus Echinostoma provides a model for future work on sibling species and will assist in the current reclassification of the group.
The Novel and Endemic Pathogen Hypotheses: Competing Explanations for the Origin of Emerging Infectious Diseases of Wildlife
Current evidence suggests chytridiomycosis may be a novel pathogen being spread worldwide by carriers; until the authors know how much genetic variation to expect in an endemic strain, however, it cannot yet conclude that B. dendrobatidis is a novel Pathogen.
A review of the application of molecular genetics for fisheries management and conservation of sharks and rays.
Increased application of the most recent and emerging technologies will enable accelerated genetic data production and the development of new markers at reduced costs, paving the way for a paradigm shift from gene to genome-scale research, and more focus on adaptive rather than just neutral variation.
Rapid Global Expansion of the Fungal Disease Chytridiomycosis into Declining and Healthy Amphibian Populations
The molecular population genetics of a global collection of fungal strains from both declining and healthy amphibian populations are addressed using DNA sequence variation from 17 nuclear loci and a large fragment from the mitochondrial genome, providing evidence that the observed genotypic variation can be generated by loss of heterozygosity through mitotic recombination.
A phylogeny of planorbid snails, with implications for the evolution of Schistosoma parasites.
Evolutionary relationships and biogeography of Biomphalaria (Gastropoda: Planorbidae) with implications regarding its role as host of the human bloodfluke, Schistosoma mansoni.
It is confirmed that a proto-Biomphalaria glabrata gave rise to all African species through a trans-Atlantic colonization of Africa, and interesting patterns occur regarding schistosome susceptibility: the most susceptible hosts belong to a single clade, and several susceptible Neotropic species are sister groups to apparently refractory species, and some basal lineages are susceptible.
Origin and diversification of the human parasite Schistosoma mansoni
The global phylogeography of S. mansoni is described using more than 2500 bp of mitochondrial DNA from 143 parasites collected in 53 geographically widespread localities, and considerable within‐species mtDNA diversity was found.
AN APPROACH TO REVEALING BLOOD FLUKE LIFE CYCLES, TAXONOMY, AND DIVERSITY: PROVISION OF KEY REFERENCE DATA INCLUDING DNA SEQUENCE FROM SINGLE LIFE CYCLE STAGES
- S. Brant, J. Morgan, G. Mkoji, S. Snyder, R. Rajapakse, E. Loker
- BiologyJournal of Parasitology
- 1 February 2006
This work provides data on the value of new information from field collections of any available life cycle stages for several blood fluke cercariae from freshwater snails from Kenya, Uganda, and Australia, and phylogenetic analyses suggest that 2 of the new schistosome specimens likely represent previously unknown lineages.