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A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions.
  • C. Cupples, J. Miller
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1 July 1989
Six strains of Escherichia coli with different mutations at the same coding position in the lacZ gene, which specifies the active site glutamic acid residue at position 461 in beta'-galactosidase, are described and useful for rapidly determining the mutagenic specificity of mutagens at a single site. Expand
Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system.
The DNA sequence changes were determined for 61 lacI mutations induced by exposure of one of the cell lines to N-nitroso-N-methylurea and provide support for the mutational theory of cancer. Expand
Evidence that MutY and MutM combine to prevent mutations by an oxidatively damaged form of guanine in DNA.
The data strongly suggest that MutY is part of an intricate repair system directed against 8-oxoG lesions in nucleic acids and that the primary function of MutY in vivo is the removal of adenines that are misincorporated opposite 8-OxoG injuries during DNA synthesis. Expand
A set of lacZ mutations in Escherichia coli that allow rapid detection of specific frameshift mutations.
A comparison of ethyl methanesulfonate, N-methyl-N'-nitro-N-nitrosoguanidine and 2-aminopurine frameshift specificity with that found with a mutH strain suggests that these mutagens partially or fully saturate or inactivate the methylation-directed mismatch repair system and allow replication errors leading to frameshifts to escape repair. Expand
Proliferation of mutators in A cell population.
A Lac- strain of Escherichia coli that reverts by the addition of a G to a G-G-G -G- g sequence was used to study the proliferation of mutators in a bacterial culture, suggesting that an early step in carcinogenesis is the creation of a mutator phenotype. Expand
A repair system for 8-oxo-7,8-dihydrodeoxyguanine.
Active oxygen species can damage DNA and may play a role in aging and carcinogenesis. We have tested MutY glycosylase for activity on undamaged mispairs as well as mispairs formed with theExpand
Spontaneous mutators in bacteria: insights into pathways of mutagenesis and repair.
  • J. Miller
  • Biology, Medicine
  • Annual review of microbiology
  • 1996
Characterizing bacterial mutators and the repair systems that are deficient can aid in discovering the human homolog of these systems, the close counterparts of the equivalent bacterial repair system. Expand
Genetic studies of the lac repressor. IV. Mutagenic specificity in the lacI gene of Escherichia coli.
The frequencies of occurrence of amber and ochre mutations arising from the G · C → A · T transition have been compared for different mutagens, revealing several striking hotspots. Expand
Cloning and sequencing a human homolog (hMYH) of the Escherichia coli mutY gene whose function is required for the repair of oxidative DNA damage.
The human mutY gene (hMYH) is cloned from both genomic and cDNA libraries and provides an important function in the repair of oxidative damage to DNA and helps to prevent mutations from oxidative lesions. Expand