J. Middleton

Publications160
h-index27
Citations2,510
Highly Influential Citations160
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Molecular Epidemiology of Mastitis Pathogens of Dairy Cattle and Comparative Relevance to Humans
Mastitis, inflammation of the mammary gland, can be caused by a wide range of organisms, including gram-negative and gram-positive bacteria, mycoplasmas and algae. Many microbial species that are… Expand
  • 254
  • 14
  • PDF
Effect of delayed colostrum collection on colostral IgG concentration in dairy cows.
OBJECTIVE To determine the effect of timing of first-milking colostrum collection on colostral IgG concentration. DESIGN Prospective study. ANIMALS 13 healthy Holstein cows. PROCEDURES All… Expand
  • 121
  • 12
Comparison of four methods to assess colostral IgG concentration in dairy cows.
OBJECTIVE To determine sensitivity and specificity of 4 methods to assess colostral IgG concentration in dairy cows and determine the optimal cutpoint for each method. DESIGN Cross-sectional study.… Expand
  • 63
  • 11
Use of somatic cell counts and California mastitis test results from individual quarter milk samples to detect subclinical intramammary infection in dairy cattle from a herd with a high bulk tank…
OBJECTIVE To determine whether somatic cell counts (SCCs) or California mastitis test (CMT) scores for individual quarter milk samples could be used to detect subclinical intramammary infection among… Expand
  • 64
  • 11
Detection of low serum immunoglobulin concentrations in clinically ill calves.
The sensitivity, specificity, and accuracy of classification of 4 tests for failure of passive transfer (FPT) were examined in clinically ill neonatal calves. Comparisons were made with serum IgG1… Expand
  • 66
  • 8
Association of coagulase-negative staphylococcal species, mammary quarter milk somatic cell count, and persistence of intramammary infection in dairy cattle.
This study was conducted to evaluate the association between subclinical intramammary infection (IMI) with coagulase-negative staphylococci (CNS), mammary quarter milk somatic cell count (SCC), and… Expand
  • 70
  • 7
Effect of colostrum administration by use of oroesophageal intubation on serum IgG concentrations in Holstein bull calves.
OBJECTIVE To determine the amount of colostral IgG required for adequate passive transfer in calves administered colostrum by use of oroesophageal intubation and evaluate the impact of other factors… Expand
  • 62
  • 6
Prevalence of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus carriage in three populations.
BACKGROUND A higher prevalence of methicillin-resistant Staphylococcus aureus (MRSA) colonization is reported in healthcare workers compared with nonhealthcare workers. HYPOTHESIS The prevalence of… Expand
  • 46
  • 6
Effect of prepartum intramammary treatment with pirlimycin hydrochloride on prevalence of early first-lactation mastitis in dairy heifers.
OBJECTIVE To determine whether prepartum intramammary treatment of dairy heifers with pirlimycin hydrochloride would reduce the prevalence of intramammary infection (IMI) and lower the somatic cell… Expand
  • 20
  • 6
Staphylococcus aureus antigens and challenges in vaccine development
  • J. Middleton
  • Biology, Medicine
  • Expert review of vaccines
  • 1 August 2008
Staphylococcus aureus is an important cause of nosocomial and community-acquired infections in humans and animals, as well as mastitis in dairy cattle. Methicillin-resistant S. aureus is increasingly… Expand
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