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DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae
Here we determine the complete genomic sequence of the Gram negative, γ-Proteobacterium Vibrio cholerae El Tor N16961 to be 4,033,460 base pairs (bp). The genome consists of two circular chromosomesExpand
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A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR.
The toxR gene of Vibrio cholerae encodes a transmembrane, DNA-binding protein that activates transcription of the cholera toxin operon and a gene (tcpA) for the major subunit of a pilus colonizationExpand
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Lysogenic Conversion by a Filamentous Phage Encoding Cholera Toxin
Vibrio cholerae, the causative agent of cholera, requires two coordinately regulated factors for full virulence: cholera toxin (CT), a potent enterotoxin, and toxin-coregulated pili (TCP), surfaceExpand
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Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system
The bacterium Vibrio cholerae, like other human pathogens that reside in environmental reservoirs, survives predation by unicellular eukaryotes. Strains of the O1 and O139 serogroups cause cholera,Expand
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Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin
Genes encoding type VI secretion systems (T6SS) are widely distributed in pathogenic Gram-negative bacterial species. In Vibrio cholerae, T6SS have been found to secrete three related proteinsExpand
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A Virulence Locus of Pseudomonas aeruginosa Encodes a Protein Secretion Apparatus
Bacterial pathogens frequently use protein secretion to mediate interactions with their hosts. Here we found that a virulence locus (HSI-I) of Pseudomonas aeruginosa encodes a protein secretionExpand
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Epidemiology, Genetics, and Ecology of ToxigenicVibrio cholerae
SUMMARY Cholera caused by toxigenic Vibrio cholerae is a major public health problem confronting developing countries, where outbreaks occur in a regular seasonal pattern and are particularlyExpand
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Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin.
The transposon TnphoA was used to generate fusions between phoA, the gene for alkaline phosphatase (PhoA), and genes encoding proteins that are secreted by Vibrio cholerae. One of the PhoA+ mutantsExpand
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Quorum-sensing regulators control virulence gene expression in Vibrio cholerae
The production of virulence factors including cholera toxin and the toxin-coregulated pilus in the human pathogen Vibrio cholerae is strongly influenced by environmental conditions. TheExpand
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Type VI secretion requires a dynamic contractile phage tail-like structure
Type VI secretion systems are bacterial virulence-associated nanomachines composed of proteins that are evolutionarily related to components of bacteriophage tails. Here we show that proteinExpand
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