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Fluorescence assay for phospholipid membrane asymmetry.
TLDR
It is demonstrated how reduction of NBD-lipids with dithionite can be used to prepare asymmetrically labeled liposomes and to measure transverse-membrane asymmetry in vesicles and the measurement of phospholipid translocase activity.
The role of aromatic side chain residues in micelle binding by pancreatic colipase. Fluorescence studies of the porcine and equine proteins.
TLDR
The emission maximum of horse colipase at 345 nm indicates a solvent-accessible tryptophan residue which becomes less so on binding of micelles, and the data are consistent with a direct insertion of dansyl-NH-tyrosine-55 into the micelle.
Physical and kinetic characterization of recombinant human cholesteryl ester transfer protein.
TLDR
Data suggest that CETP has a non-spheroidal shape in solution, and in dual-label transfer experiments, the transfer rates for CE or TG from HDL to LDL were found to be proportional to the initial concentrations of the respective ligands in the donor HDL particles.
Synthesis and characterization of the dansyltyrosine derivatives of porcine pancreatic colipase.
TLDR
Experiments showed that DNStyr was a sensitive probe of solvent polarity and viscosity, but not of pH, and showed that Tyr55 was located on the solvent-exposed surface of the protein, where the fluorophore experienced free rotation.
The hypocholesterolaemic effects of sitostanol in the guinea pig are in part related to changes in hepatic lipids and lipoprotein composition.
TLDR
The results suggest that SI might have its hypocholesterolaemic effect by reducing cholesterol absorption, which results in lower concentration of cholesterol in liver, which might possibly alter hepatic cholesterol metabolism and affect lipoprotein concentration and composition.
Large-scale preparation of asymmetrically labeled fluorescent lipid vesicles.
TLDR
A method for producing lipid vesicles containing fluorescent phospholipid analogues localized to the inner leaflet of their membrane was developed and can be used to produce large amounts of asymmetrically labeled liposomes suitable for use in investigating a variety of membrane phenomena.
Corn husk oil lowers plasma LDL cholesterol concentrations by decreasing cholesterol absorption and altering hepatic cholesterol metabolism in guinea pigs.
TLDR
The data from these studies suggest that CoHO has its hypocholesterolemic effect by decreasing cholesterol absorption and increasing bile acid output and these alterations in the intestinal lumen alter hepatic cholesterol metabolism and may affect the synthesis and catabolism of lipoproteins.
Corn fiber oil lowers plasma cholesterol by altering hepatic cholesterol metabolism and up-regulating LDL receptors in guinea pigs.
TLDR
The results suggest that CFO, as a result of decreased bile acid absorption, increased mRNA abundance and activity of CYP7, is suggested to be responsible for the observed decrease in plasma LDL cholesterol concentrations.
The interaction of bile salt micelles with the dansyltyrosine derivatives of porcine colipase.
The interaction of bile salt micelles with the tyrosines of pancreatic colipase was assessed by steady-state and time-resolved fluorescence techniques. Dansyltyrosine fluorescence showed that Tyr-55
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