Share This Author
New ways to break an old bond: the bacterial carbon-phosphorus hydrolases and their role in biogeochemical phosphorus cycling.
Whole-genome and metagenome sequence analysis indicates that the genes encoding these newly described C-P hydrolases are distributed widely among prokaryotes, and may play a hitherto-unrecognized role in phosphonate breakdown in the environment and hence make a significant contribution to global biogeochemical P-cycling.
Development and application of a resazurin-based biomass activity test for activated sludge plant management.
Organophosphonates revealed: new insights into the microbial metabolism of ancient molecules
Advances in the understanding of the biochemistry and genetics of microbial phosphonate metabolism are reviewed, and the role of these compounds and of the organisms engaged in their turnover within the P cycle are discussed.
Predicted Relative Metabolomic Turnover (PRMT): determining metabolic turnover from a coastal marine metagenomic dataset
The PRMT method was successfully applied to metagenomic data to explore the Western English Channel microbial metabalome to generate specific, biologically testable hypotheses and can be applied as a general tool for the analysis of additional meetagenomic or transcriptomic datasets.
Metagenomic Characterisation of the Viral Community of Lough Neagh, the Largest Freshwater Lake in Ireland
Analysis of total metagenomic DNA from the water column of Lough Neagh has revealed a high proportion of Cyanobacteria and low levels of Actinob bacteria, Acidobacteria, Chloroflexi, and Firmicutes.
Intracellular Accumulation of Polyphosphate by the Yeast Candida humicola G-1 in Response to Acid pH
- J. McGrath, J. Quinn
- Biology, Environmental ScienceApplied and Environmental Microbiology
- 1 September 2000
ABSTRACT Cells of a newly isolated environmental strain of Candida humicola accumulated 10-fold more polyphosphate (polyP), during active growth, when grown in complete glucose-mineral salts medium…
Hydrophobic substances induce water stress in microbial cells
- Prashanth Bhaganna, Rita. J. M. Volkers, J. E. Hallsworth
- BiologyMicrobial Biotechnology
- 22 October 2010
It is suggested that it may be possible to devise new interventions for microbial processes in both natural environments and industrial reactors to expand microbial tolerance of hydrophobic substances, and hence the biotic windows for such processes.
The purification and properties of phosphonoacetate hydrolase, a novel carbon-phosphorus bond-cleavage enzyme from Pseudomonas fluorescens 23F.
- J. McGrath, G. B. Wisdom, G. McMullan, M. Larkin, J. Quinn
- BiologyEuropean journal of biochemistry
- 1 November 1995
A novel, inducible, carbon-phosphorus bond-cleavage enzyme, phosphonoacetate hydrolase, was purified from cells of Pseudomonas fluorescens 23F grown on phosphono acetate and displayed no significant similarity to other databank sequences.
The Genes and Enzymes of Phosphonate Metabolism by Bacteria, and Their Distribution in the Marine Environment
The data presented indicate the likely importance of phosphonate-P in global biogeochemical P cycling, and by extension its role in marine productivity and in carbon and nitrogen dynamics in the oceans.
An Evaluation of Three New-Generation Tetrazolium Salts for the Measurement of Respiratory Activity in Activated Sludge Microorganisms
XTT-formazan production was found to be stimulated by the availability of an exogenous carbon and energy source, and by the presence of the electron-coupling agent phenazine methosulfate, and less than 3% of XTT reduction by an activated sludge sample was abiotic.