J. McCafferty

Publications97
h-index32
Citations9,628
Highly Influential Citations272
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By-passing immunization. Human antibodies from V-gene libraries displayed on phage.
TLDR
The results suggest that a single large phage display library can be used to isolate human antibodies against any antigen, by-passing both hybridoma technology and immunization. Expand
Quantitative visualization of DNA G-quadruplex structures in human cells.
TLDR
It is shown explicitly that G-quadruplex formation in DNA is modulated during cell-cycle progression and that endogenous G- quadruplex DNA structures can be stabilized by a small-molecule ligand and corroborate the application of stabilizing ligands in a cellular context to target G- Quadruplexes and intervene with their function. Expand
Phage antibodies: filamentous phage displaying antibody variable domains
TLDR
It is shown that complete antibody V domains can be displayed on the surface of fd bacteriophage, that the phage bind specifically to antigen and that rare phage can be isolated after affinity chromatography. Expand
Human Antibodies with Sub-nanomolar Affinities Isolated from a Large Non-immunized Phage Display Library
TLDR
This work shows that conventional hybridoma technology may be superseded by large phage libraries that are proving to be a stable and reliable source of specific, high affinity human monoclonal antibodies. Expand
Phage display of peptides and proteins : a laboratory manual
TLDR
The filamentous bacteriophage is a cDNA cloning system based on filamentous phage - selection and enrichment of functional gene products by protein/ligand interactions made possible by linkage of recognition and replication functions. Expand
Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression
TLDR
Analysis of the protein features identified here will help predict which mammalian proteins and domains can be successfully expressed in E. coli as soluble product and also which are best targeted for a eukaryotic expression system. Expand
Application of phage display to high throughput antibody generation and characterization
TLDR
The potential of and the issues associated with genome-wide monoclonal antibody generation are demonstrated and illuminated by a high quality phage display library containing over 1010 human antibodies. Expand
Thiol isomerases negatively regulate the cellular shedding activity of ADAM17.
TLDR
This work has utilized a cell-based ADAM17 assay to show that thiol isomerases, specifically PDI (protein disulfide isomerase), could be responsible for maintainingADAM17 in an inactive form. Expand
Beyond natural antibodies: the power of in vitro display technologies
TLDR
The amenability of in vitro display to high-throughput applications broadens the prospects for their wider use in basic and applied research. Expand
Cross-domain inhibition of TACE ectodomain
TLDR
The bespoke development of a specific TACE inhibitory human antibody using “two-step” phage display is reported, which is a previously undescribed selective TACE antagonist and provides a unique alternative to small-molecule metalloprotease inhibition. Expand
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