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Characterization of a B. subtilis minor isoleucine tRNA deduced from tDNA having a methionine anticodon CAT.
TLDR
This tRNA transcribed from the gene possessing the CAT anticodon agrees with the minor one predicted from the DNA sequence and is thought to decode the isoleucine codon AUA.
Effect of B. subtilis TRNA(Trp) on readthrough rate at an opal UGA codon.
TLDR
It is suggested that this tRNATrp has a dual recognition pattern in B. subtilis, i.e., for the canonical tryptophan codon and for readthrough at the UGA stop codon.
Oncogenic Ras mutant causes the hyperactivation of NF‐κB via acceleration of its transcriptional activation
TLDR
It is suggested that oncogenic signal‐induced acceleration of NF‐κB activation is caused by activation of the p38 MAP kinase–MSK1 signaling axis and by cell cycle progression in cancer cells.
Modification of the anticodon triplet of E.coli tRNAMetf by replacement with trimers complementary to non-sense codons UAG and UAA.
TLDR
E. coli tRNAMetf was hydrolyzed with RNase A to give two half molecules lacking the anticodon trimer and 3'-terminal dimer, and it was found that all three modified molecules were not recognized by the methionyl-tRNA synthetase from E.coli.
Modification of the amino acid acceptor stem of E. coli tRNAf Met by ligation of chemically synthesized ribooligonucleotides
TLDR
The single‐stranged region of the amino acid acceptor stem corresponding to the 3'‐end of E. coli tRNAMet f was replaced by ligation of chemically synthesized ribooligonucleotides, in order to change the length of the single‐Stranded CCA terminus, which restored the methionine acceptor activity.
Replacement and insertion of nucleotides at the anticodon loop of E. coli tRNAMetf by ligation of chemically synthesized ribooligonucleotides.
TLDR
The presence of the 3'-side of the anticodon loop of tRNA is one of the major recognition sites for the methionyl-tRNA synthetase in the E. coli tRNAMetf molecule tested for their ability to accept methionine and the affinity of these analogs for the Synthetase decreased more extensively when the insertion was at the 3-side ofThe anticodon triplet.
ST2 gene products critically contribute to cellular transformation caused by an oncogenic Ras mutant
TLDR
It was indicated that the oncogenic Ras-induced expression of ST2 gene products is required for the acceleration of cellular transformation, and this seems to be independent of the stimulation with IL-33, a ligand for ST2/ST2L.
Nucleotide sequences of serine tRNAs from Bacillus subtilis.
TLDR
Three B. subitilis serine tRNAs were sequenced including modified nucleosides, and 5-methoxyuridine was found in the first letter of the anticodon in the tRNA(UGA).
Biochemical properties of Caenorhabditis elegans HMG-5, a regulator of mitochondrial DNA.
TLDR
It is found that knockdown of HMG-5 reduced the amount of mtDNA in P0 hermaphrodites, suggesting it as functional orthologue of mammalian TFAM, and in vitro binding experiment showed that H MG-5 binds to C. elegans mtDNA and plasmid DNA, indicating its feature as a non-specific DNA-binding protein.
tRNA1Ser(G34) with the anticodon GGA can recognize not only UCC and UCU codons but also UCA and UCG codons.
TLDR
The tRNA1Ser(G34) having the anticodon GGA was able to read not only UCC and UCU codons but also UCA and UCG codons, and depended on the 2'-O-methylation of the C32 (Cm) for translational efficiency.
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