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Molecular characterization of the plasmid‐encoded eps gene cluster essential for exopolysaccharide biosynthesis in Lactococcus lactis
Lactococcus lactis strain NIZO B40 produces an extracellular phosphopolysaccharide containing galactose, glucose, and rhamnose. A 40 kb plasmid encoding exopolysaccharide production was isolatedExpand
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Medium-dependent regulation of proteinase gene expression in Lactococcus lactis: control of transcription initiation by specific dipeptides.
Transcriptional gene fusions with the Escherichia coli beta-glucuronidase gene (gusA) were used to study the medium- and growth-dependent expression of the divergently transcribed genes involved inExpand
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Molecular and Functional Analyses of themetC Gene of Lactococcus lactis, Encoding Cystathionine β-Lyase
ABSTRACT The enzymatic degradation of amino acids in cheese is believed to generate aroma compounds and therefore to be essential for flavor development. Cystathionine β-lyase (CBL) can convertExpand
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The ferric‐pseudobactin receptor PupA of Pseudomonas putida WCS358: homology to TonB‐dependent Escherichia coli receptors and specificity of the protein
The initial step in the uptake of iron via ferric pseudobactin by the plant‐growth‐promoting Pseudomonas putida strain WCS358 is binding to a specific outer‐membrane protein. The nucleotide sequenceExpand
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Isolation and analysis of genes involved in siderophore biosynthesis in plant-growth-stimulating Pseudomonas putida WCS358.
The plant-growth-stimulating Pseudomonas putida WCS358 was mutagenized with transposon Tn5. The resulting mutant colony bank was screened for mutants defective in the biosynthesis of the fluorescentExpand
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Specialized Roles of the Two Pathways for the Synthesis of Mannosylglycerate in Osmoadaptation and Thermoadaptation of Rhodothermus marinus*
Rhodothermus marinus responds to fluctuations in the growth temperature and/or salinity by accumulating mannosylglycerate (MG). Two alternative pathways for the synthesis of MG have been identifiedExpand
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Regulation of Proteolytic Enzyme Activity in Lactococcus lactis.
Two different Lactococcus lactis host strains, L. lactis subsp. lactis MG1363 and L. lactis subsp. cremoris SK1128, both containing plasmid pNZ521, which encodes the extracellular serine proteinaseExpand
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A simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples.
A method was developed to detect and identify Enterobacter sakazakii in environmental samples. The method is based on selective enrichment at 45+/-0.5 degrees C in lauryl sulfate tryptose brothExpand
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Exopolysaccharide biosynthesis in Lactococcus lactis NIZO B40: functional analysis of the glycosyltransferase genes involved in synthesis of the polysaccharide backbone.
We used homologous and heterologous expression of the glycosyltransferase genes of the Lactococcus lactis NIZO B40 eps gene cluster to determine the activity and substrate specificities of theExpand
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The role of siderophores in potato tuber yield increase by Pseudomonas putida in a short rotation of potato
The effect of treatment of potato seed tubers withPseudomonas putida isolate WCS358 on tuber yield was studied in different crop rotations at the Experimental Farm ‘De Schreef’, near Lelystad. WithExpand
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