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Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum
Genes predicted to encode transporters were strongly overrepresented among the genes transcriptionally upregulated under conditions that stimulate penicillinG production, illustrating potential for future genomics-driven metabolic engineering. Expand
Phosphate Control of the Biosynthesis of Antibiotics and Other Secondary Metabolites Is Mediated by the PhoR-PhoP System: an Unfinished Story
  • J. Martín
  • Medicine, Biology
  • Journal of bacteriology
  • 15 August 2004
Directed phoR-phoP gene disruption will be very useful for the construction of tailored phosphate-deregulated strains overproducing valuable secondary metabolites. Expand
The dynamic architecture of the metabolic switch in Streptomyces coelicolor
This study provides the first detailed insights into the complex sequence of early regulatory events during and preceding the major metabolic switch in S. coelicolor, which will form the starting point for future attempts at engineering antibiotic production in a biotechnological setting. Expand
Binding of PhoP to promoters of phosphate‐regulated genes in Streptomyces coelicolor: identification of PHO boxes
The control of phosphate‐regulated genes in Streptomyces coelicolor is mediated by the two‐component system PhoR–PhoP. When coupled to the reporter xylE gene the pstS, phoRP and phoU promoters wereExpand
Cross‐talk between two global regulators in Streptomyces: PhoP and AfsR interact in the control of afsS, pstS and phoRP transcription
The reciprocal regulation of the phoRP promoter by AfsR and of afsS by PhoP suggests a fine interplay of these regulators on the control of secondary metabolism. Expand
Amplification and disruption of the phenylacetyl-CoA ligase gene of Penicillium chrysogenum encoding an aryl-capping enzyme that supplies phenylacetic acid to the isopenicillin N-acyltransferase.
Peroxisomal co-localization of these two proteins indicates that the last two enzymes of the penicillin pathway form a peroxisome-targeting sequence that is also present in the isopenicillin N acyltransferase. Expand
PimM, a PAS domain positive regulator of pimaricin biosynthesis in Streptomyces natalensis.
Results indicate that PimM plays its regulatory role independently of PimR, the first pathway-specific regulator of pimaricin biosynthesis, and the genes responsible for initiation and first elongation cycles of polyketide chain extension are among the major targets for regulation. Expand
Stable transformants of the azaphilone pigment-producing Monascus purpureus obtained by protoplast transformation and Agrobacterium-mediated DNA transfer
The high-level pigment-producing Monascus strain IBCC1 was characterized by random amplification of polymorphic DNA as M. purpureus and the transformants obtained by Agrobacterium-mediated DNA transfer remained fully stable after four sporulation rounds and showed bands of hybridization corresponding to integration of the plasmid in different sites of the genome. Expand
Characterization of an Endogenous Plasmid and Development of Cloning Vectors and a Transformation System in Brevibacterium lactofermentum
A cryptic plasmid, pBL1 of 4.3 kb, has been found in lysine-producing Brevibacterium lactofermentum strains BL0, BL70, BL74 and BL77 and a shuttle vector able to replicate in Escherichia coli, Streptomyces lividans and B. lactfermentum protoplasts was developed. Expand
Identification of PimR as a Positive Regulator of Pimaricin Biosynthesis in Streptomyces natalensis
Gene expression analysis by reverse transcriptase PCR of the pimaricin gene cluster revealed that S. natalensis DeltaPimR shows no expression at all of the cholesterol oxidase-encoding gene pimE, and very low level transcription of the remaining genes of the cluster except for the mutant pimR gene, demonstrating that this regulator activates the transcription of all the genes belonging to the pimAricin genes but not its own transcription. Expand