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High resolution 31P nuclear magnetic resonance studies of intact yeast cells.
High resolution 31P nuclear magnetic resonance (NMR) spectra at 145.7 MHZ are presented for intact yeast cells. Several peaks are resolved and assigned. They include the middle phosphate peaks fromExpand
Kinetics of reaction of nitrite with deoxy hemoglobin after rapid deoxygenation or predeoxygenation by dithionite measured in solution and bound to the cytoplasmic domain of band 3 (SLC4A1).
The reaction of deoxyhemoglobin with nitrite was characterized in the presence of dithionite using hemoglobin in solution or bound to the cytoplasmic domain of band 3 (CDB3). Deoxyhemoglobin wasExpand
Erythrocyte Band 3 Protein
Kinetics of p-mercuribenzoate binding to sulfhydryl groups on the isolated cytoplasmic fragment of band 3 protein. Effect of hemoglobin binding on the conformation.
Hemoglobin binds to the cytoplasmic domain of band 3 protein (CDB3) at physiologic pH and ionic strength in an oxygen-linked fashion, with deoxyhemoglobin having the higher affinity. The evidence inExpand
Direct generation of superoxide anions by flash photolysis of human oxyhemoglobin.
The results presented in this report suggest that human oxyhemoglobin can directly form methemoglobin and superoxide anion when flashed with low intensity (38 joules) white light. The effect onlyExpand
Lipid-mediated impairment of normal energy metabolism in the isolated perfused diabetic rat heart studied by phosphorus-31 NMR and chemical extraction.
The relationship between extracellular palmitate and the accumulation of long-chain fatty-acyl coenzyme A with that of high-energy phosphate metabolism was investigated in the isolated perfusedExpand
Measurement of free magnesium in perfused and ischemic arrested heart muscle. A quantitative phosphorus-31 nuclear magnetic resonance and multiequilibria analysis.
The concentration of intracellular free Mg2+ in perfused, beating guinea pig hearts has been estimated on the basis of the Mg2+-dependent separation between the alpha- and beta-phosphorus resonancesExpand
The effects of 2,3-diphosphoglycerate on the kinetics of deoxygenation of human hemoglobin.
Abstract Using a stopped flow apparatus, the effects of 2,3-diphosphoglycerate (DPG), and pH on the hemoglobin-oxygen dissociation kinetic constants were determined. The order of kinetics wasExpand
Characterization of the pH dependence of hemoglobin binding to band 3. Evidence for a pH-dependent conformational change within the hemoglobin-band 3 complex.
The pH dependence of hemoglobin binding to inside-out red cell membrane vesicles was studied using 90 degrees light scattering (Salhany, J.M. et al., Biochemistry 19 (1980) 1447-1454). HyperbolicExpand