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Cloning and characterisation of genes for tetrapyrrole biosynthesis from the cyanobacterium Anacystis nidulans R2
TLDR
The genes for 5-aminolevulinic acid dehydratase (ALAD) and uroporphyrinogen III synthase (UROS) were independently isolated from a plasmid-based genomic library of Anacystis nidulans R2 by their ability to complement Escherichia coli strains carrying mutations in the equivalent genes (hemB and hemD respectively).