• Publications
  • Influence
Plasmid and chromosome partitioning: surprises from phylogeny
TLDR
A phylogenetic analysis of par loci from plasmids and chromosomes from prokaryotic organisms finds a new par gene family from enteric bacteria that is consistent with lateral gene transfer between Bacteria and Archaea.
Dysfunctional MreB inhibits chromosome segregation in Escherichia coli
TLDR
Flow cytometric and cytological analyses indicated that MreB‐depleted cells segregated their chromosomes in pairs, consistent with chromosome cohesion, and observations indicate that M reB filaments participate in directional chromosome movement and segregation.
Prokaryotic DNA segregation by an actin‐like filament
TLDR
In vivo and in vitro results indicate that ParM polymerization generates the force required for directional movement of plasmids to opposite cell poles and that the ParR–parC complex functions as a nucleation point for ParM Polymerization.
Bacterial mitosis: ParM of plasmid R1 moves plasmid DNA by an actin-like insertional polymerization mechanism.
TLDR
The mechanism by which the three components of the R1 par system act together to generate the force required for plasmid movement during segregation in E. coli appears to be mechanistically analogous to the actin-based motility operating in eukaryotic cells.
Novel coiled‐coil cell division factor ZapB stimulates Z ring assembly and cell division
TLDR
The crystal structure showed that ZapB exists as a dimer that is 100% coiled‐coil, and time lapse microscopy showedthat ZapB–GFP is present at mid‐cell in a pattern very similar to that of FtsZ, raising the possibility that Zap B stimulates Z ring formation directly via its capacity to self‐assemble into larger structures.
Switching off small RNA regulation with trap‐mRNA
TLDR
It is shown that MicM, which silences the expression of an outer membrane protein, YbfM under most growth conditions, does not become destabilized by target mRNA overexpression, indicating that the small RNA regulator acts catalytically.
F‐actin‐like filaments formed by plasmid segregation protein ParM
TLDR
It is shown here that ParM polymerizes into double helical protofilaments with a longitudinal repeat similar to filamentous actin (F‐actin) and MreB filaments that maintain the cell shape of non‐spherical bacteria.
Towards understanding the molecular basis of bacterial DNA segregation
TLDR
The molecules and mechanisms that govern the correct subcellular positioning and rapid separation of newly replicated chromosomes and plasmids towards the cell poles are reviewed and the emergence of mitotic-like machineries capable of segregating plasmid DNA is described.
Translational Regulation of Gene Expression by an Anaerobically Induced Small Non-coding RNA in Escherichia coli*
TLDR
The identification and characterization of a highly conserved, anaerobically induced small sRNA in E. coli provides insight into the mechanism by which FNR negatively regulates genes such as sodA, sodB, cydDC, and metE, thereby demonstrating that adaptation to anaerobic growth involves the action of a small regulatory RNA.
Structural analysis of the ParR/parC plasmid partition complex
TLDR
Crystallographic and electron microscopic data indicate that ParR dimers assemble into a helix structure with DNA‐binding sites facing outward, which holds implications for how ParM polymerization drives active DNA transport during plasmid partition.
...
1
2
3
4
5
...