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Replicase Complex Genes of Semliki Forest Virus Confer Lethal Neurovirulence
ABSTRACT Semliki Forest virus (SFV) is a mosquito-transmitted pathogen of small rodents, and infection of adult mice with SFV4, a neurovirulent strain of SFV, leads to lethal encephalitis in a fewExpand
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Rhizavidin from Rhizobium etli: the first natural dimer in the avidin protein family.
Rhizobium etli CFN42 is a symbiotic nitrogen-fixing bacterium of the common bean Phaseolus vulgaris. The symbiotic plasmid p42d of R. etli comprises a gene encoding a putative (strept)avidin-likeExpand
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Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli.
Chicken avidin is a highly popular tool with countless applications in the life sciences. In the present study, an efficient method for producing avidin protein in the periplasmic space ofExpand
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Crystal structure of rhizavidin: insights into the enigmatic high-affinity interaction of an innate biotin-binding protein dimer.
Rhizavidin, from the proteobacterium Rhizobium etli, exhibits high affinity towards biotin but maintains an inherent dimeric quaternary structure and thus, differs from all other known tetramericExpand
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Avidin related protein 2 shows unique structural and functional features among the avidin protein family
BackgroundThe chicken avidin gene family consists of avidin and several avidin related genes (AVRs). Of these gene products, avidin is the best characterized and is known for its extremely highExpand
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Detection of myelin basic protein isoforms by organic concentration.
An effective technique was developed, which allowed rapid isolation of highly pure myelin basic protein (MBP) including its distinct isoforms. The procedure employs homogenization of central nervousExpand
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Design and Construction of Highly Stable, Protease-resistant Chimeric Avidins*
The chicken avidin gene family consists of avidin and seven separate avidin-related genes (AVRs) 1–7. Avidin protein is a widely used biochemical tool, whereas the other family members have onlyExpand
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Chemokine expression by central nervous system resident cells and infiltrating neutrophils during experimental autoimmune encephalomyelitis in the BALB / c mouse
The active role of chemokines in the central nervous system (CNS) during the pathogenesis of experimental autoimmune encephalomyelitis (EAE) has been clearly established. In this study the expressionExpand
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The Semliki Forest virus E2 gene as a virulence determinant.
We have determined the nucleotide sequences of the capsid, E3, E2 and 6K genes of the avirulent Semliki Forest virus variant A774 (SFV A7). The sequence analysis revealed a nucleotide identity of 98%Expand
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Tetravalent single-chain avidin: from subunits to protein domains via circularly permuted avidins.
scAvd (single-chain avidin, where two dcAvd are joined in a single polypeptide chain), having four biotin-binding domains, was constructed by fusion of topologically modified avidin units. scAvdExpand
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